The Brilliant Cresyl Blue (BCB) test relies on G6PDH activity and a simple protocol for the selection of higher quality oocytes. Although the BCB+ oocytes of all the species that have been investigated are characterized by superior quality when compared to BCB- counterparts, application of the test for embryo production still remains an open issue. The aim of our study was to compare BCB+ and t...

The aim of this study was to assess the effect of oocyte selection using the brilliant cresyl blue (BCB) test plus the addition of cysteamine to the in vitro maturation (IVM) medium to improve the in vitro embryo development of prepubertal goat oocytes. The oocytes were exposed to 26 microM BCB and classified according to their cytoplasm coloration: BCB+ (oocytes with blue cytoplasm) and BCB- (...

Reticulocytes are precursory to the erythrocyte cells in the blood. They are anucleated cells, more rounded in shape and 20% greater, in volume, than the erythrocytes. However, when stained with panoptic dyes (Romanowski) they produce polychromatic slides, due to the presence of mature red blood cells with hemoglobin, synthesized during maturation, and reticulocytes with ribonucleic acid residu...

22 23 The gGlycolysis and the pentose phosphate pathway (PPP) were modulated in porcine 24 cumulus-oocyte complexes (COCs) during in vitro maturation (IVM) by the addition of 25 inhibitors and stimulators of key enzymes of the pathways, with the aim of to analizing 26 analyze their participation influence on the oxidative status, mitochondrial activity and 27 maturation of the oocyte. The influ...

When living cells of Nitella are exposed to a solution of sodium acetate and are then placed in a solution of brilliant cresyl blue made up with a borate buffer mixture at pH 7.85, a decrease in the rate of penetration of dye is found, without any change in the pH value of the sap. It is assumed that this inhibiting effect is caused by the action of sodium on the protoplasm. This effect is not ...

An adequate theory of the penetration 1,~,3 of cresyl blue into the vacuole of living cells of the fresh water plant Nitella may be stated as follows. The dye, in form of free base, which predominates at a high pH value, diffuses in and out of the vacuole very freely, while the dye in form of salt, which predominates at a low pH value, diffuses so slowly that its rate of penetration and that of...