In synchronized continuous cultures of Saccharomyces cerevisiae CBS 8066, the production of the extracellular invertase (EC 3.2.1.26) showed a cyclic behavior that coincided with the budding cycle. The invertase activity increased during bud development and ceased at bud maturation and cell scission. The cyclic changes in invertase production resulted in cyclic changes in amounts of invertase l...

A simple method is described for growing rat embryos in vitro for 48 hr from head-fold to early limb-bud stages at rates of development and protein synthesis indistinguishable from those in vivo. Culture of the embryos can be continued for longer periods but at a reduced growth rate. Preheating the culture serum to 56 degrees C for 30 min improves embryonic development, but raising the culture ...

Antagonists act to restrict and negatively modulate the activity of secreted signals during progression of embryogenesis. In mouse embryos lacking the extra-cellular BMP antagonist gremlin 1 (Grem1), metanephric development is disrupted at the stage of initiating ureteric bud outgrowth. Treatment of mutant kidney rudiments in culture with recombinant gremlin 1 protein induces additional epithel...

During vertebrate embryogenesis, the muscle-specific helix-loop-helix protein myogenin is expressed in muscle cell precursors in the developing somite myotome and limb bud before muscle fiber formation and is further upregulated during myogenesis. We show that cis-acting DNA sequences within the 5' flanking region of the mouse myogenin gene are sufficient to direct appropriate temporal, spatial...

Chlorophytum arundinaceum is an important medicinal plant and its tuberous roots are used for various health ailment treatments. It has become an endangered species in the Eastern Ghats, and a rare medicinal herb in India, due to its excessive collection from its natural habitat and its destructive harvesting techniques, coupled with poor seed germination and low vegetative multiplication ratio...

We report development of a model of retroviral gene transduction in high-density limb bud cell micromass culture. The replication competent avian retrovirus RCAS BP (A) carrying the human placental alkaline phosphatase gene (RCAS AP) was used as a marker for retroviral infection and spread. The final protocol balances the need to allow time for retroviral integration and gene transduction again...

The initiation of limb development depends on the site specific proliferation of the mesenchyme by the signals from the apical ectodermal ridge (AER) in embryonic mouse. We have previously reported that the local expression of Hst-1/Fgf-4 transcripts in AER of the mouse limb bud is developmentally regulated, expressed at 11 and 12 days post coitus (p.c.) embryo. In an effort to further understa...

In Ayurveda, Blumea lacera extraction has been found cast-off in the management of various sympathetic diseases and is also to exhibit hypoglycaemic, anti-diarrhoeal, larvicidal, antioxidant antimicrobial properties. contain astringent, stomachic, antipyretic, diuretic, cure bronchitis fever. To find out most suitable part plant which will be used for killing mosquito larvae. How do produce eff...

due to physiological disorder, primary bud necrosis (pbn) in grapevine is a great problem in vineyards, resulting in reduced yield. primary bud necrosis (pbn) is physiological disorder resulting in the death of the primary bud. the present study conducted through a light on this critical problem in a vineyard of cisakht region. a factorial experiment with three levels of cold temperature (-10, ...

phosphorus induces flowering and helps seed formation, but there is a big gap between phosphorus supply from natural sources and agriculture and industry demand. in order to investigate phosphorus feeding over long time from iran regions' apatites on rose plants characteristics, var. black magic in zeoponic culture, an experiment was conducted as factorial on the basis of completely random...