Abstract Background Cucumber ( Cucumis sativus L.) is cultivated worldwide, and it essential to produce enough high-quality seeds meet demand. Pre-harvest sprouting (PHS) in cucumber a critical problem causes serious damage seed production quality. Nevertheless, the genetic basis molecular mechanisms underlying PHS remain unclear. QTL-seq an efficient approach for rapid quantitative trait loci ...

Bulked segregant analysis was used to determine randomly amplified polymorphic DNA (RAPD) markers in a specific interval in the middle of chromosome 6 of rice for tagging the photoperiod sensitivity gene. Two pools of F2 individuals (japonica cv. Nipponbare and indica cv. Kasalath) were constructed according to the genotypes of three restriction fragment length polymorphism (RFLP) markers locat...

Nitrogen is a major nutritional element in rice production. However, excessive application of nitrogen fertilizer has caused severe environmental pollution. Therefore, development of rice varieties with improved nitrogen use efficiency (NUE) is urgent for sustainable agriculture. In this study, bulked segregant analysis (BSA) combined with whole genome re-sequencing (WGS) technology was applied...

Amplified fragment length polymorphism (AFLP) analysis was used in combination with bulked segregant analysis (BSA) to identify molecular markers linked to two cowpea (Vigna unguiculata (L.) Walp.) genes conferring resistance to Striga gesnerioides race 1. After AFLP analysis of an F2 population derived from a cross between the resistant cultivar Gorom and the susceptible cultivar Tvx 3236, sev...

High-temperature stress can cause serious abiotic damage that limits the yield and quality of rice. Heat tolerance (HT) during flowering stage rice is a key trait guarantee high stable under heat stress. HT complex regulated by multiple quantitative loci (QTLs); however, few underlying genes have been fine mapped cloned. In this study, F 2:3 population derived from cross between Huanghuazhan (H...

Spike length (SL) and spike compactness (SC) are crucial traits related to wheat ( Triticum aestivum L.) yield potential. In this study, a backcrossed inbred lines (BILs) population segregating for SL/SC was developed by using commercial variety chuanyu25 as recurrent parent backbone Chuanyu12D7. Bulked segregant analysis (BSA) combined with the Wheat 660K SNP array performed conduct quantitati...

Abstract Background Plant height is an important plant characteristic closely related to yield performance of many crops. Reasonable reduction crops beneficial for improving and enhancing lodging resistance. Results In the present study, we described Brassica napus dwarf mutant bnd2 that was isolated using ethyl methanesulfonate (EMS) mutagenesis. Compared wild type (WT), exhibited reduced shor...

Both snail and parasite genes determine the susceptibility of the snail Biomphalaria glabrata to infection with the trematode Schistosoma mansoni. To identify molecular markers associated with resistance to the parasite in the snail host, we performed genetic crosses between parasite-resistant and -susceptible isogenic snails. Because resistance to infection in adult snails is controlled by a s...

Zinc (Zn) is an essential element for plant growth and development, and Zn derived from crop plants in the diet is also important for human health. Here, we report that genetic variation in Heavy Metal-ATPase 4 (HMA4) controls natural variation in leaf Zn content. Investigation of the natural variation in leaf Zn content in a world-wide collection of 349 Arabidopsis thaliana wild collected acce...