The present study aimed to explore the factors that affect polymerase chain reaction using sequence-specific primers (PCR-SSP) and to establish an optimized PCR-SSP method for detecting multiple gene polymorphisms simultaneously. The amplification system parameters, including the concentrations of Mg2+, dNTPs, pfu Taq, primers and control primers, were optimized using the designed PCR-SSP react...

Thirteen species of benthic marine algae are reported new for the isolated mid-Atlantic Azores archipelago and five older records are confirmed. Seven (Bangia atropurpurea, Codium fragile spp. tomentosoides, Ectocarpus fasciculatus, E. siliculosus, Porphyrostromium ciliare, Ulothrix flacca and Ulvaria oxysperma) are widespread amphi-Atlantic species some of which extend in distributional range ...

Se evaluó la calidad de semilla sexual papa (SSP) híbrida (Solanum tuberosum L.) cinco progenies que pueden ser utilizadas en producción alimento destinado al consumo humano. Para evaluar estas híbridas SSP se usaron diferentes parámetros indicadores semilla: a) viabilidad, b) germinación a 20 °C, c) 27 °C/40 d) tiempo medio germinación, y e) velocidad germinación. La hipótesis fue c...

In the present study, we examined the role of the neurokinin-1 receptor (NK1R) in the modulation of respiratory rhythm in a functionally identified bradypnoeic region of the ventral respiratory group (VRG) in the in situ arterially perfused juvenile rat preparation. In electrophysiologically and functionally identified bradypnoeic sites corresponding to the Bötzinger complex (BötC), microinject...

Background: The vegetables provide important nutrients to human beings. Nevertheless, contaminated vegetables can cause health problems because of their microbial load. The aim of this study was to assess the microbial quality of three main leafy vegetables cultivated and consumed at Porto-Novo in Republic of Benin. Methods: Totally, 36 samples of amaranth, nightshade, and lettuce were taken f...

Abstract Many tumour cells show dependence on exogenous serine and dietary glycine starvation can inhibit the growth of these cancers extend survival in mice. However, numerous mechanisms promote resistance to this therapeutic approach, including enhanced expression de novo synthesis pathway (SSP) enzymes or activation oncogenes that drive synthesis. Here we inhibition PHGDH, first step SSP, co...

Fungal secondary compounds and total extracts are known to affect growth of bacteria, fungi, and plants. This study tested the effects of purified compounds and total extracts from three lichens on the growth of two plant pathogens, Ophiostoma novo-ulmi ssp. americana and Sclerotinia sclerotiorum. Usnic acid showed no reduction in relative growth rates (RGR), whereas vulpinic acid reduced RGR f...

Neurokinins, such as substance P (SP), modulate the reflex regulation of cardiovascular and respiratory function in the CNS, particularly in the nucleus tractus solitarius (NTS). There is considerable evidence of the action of SP in the NTS, but the precise effects have not yet been determined. Voltage-dependent Ca2+ channels (VDCCs) serve as crucial mediators of membrane excitability and Ca2+ ...

Analysis of non-halal components, such as pork and porcine gelatin, in food pharmaceutical products is a need for halal authentication study. This research was aimed to develop species-specific primer (SSP) analyze DNA gelatin soft candy using real-time PCR. The SSP designed NCBI Primer-BLAST software. subjected validation by assessing some parameters, including specificity, sensitivity, repeat...