A novel strategy for immunoassay and the preparation of reagentless immunosensors was proposed. This strategy was based on the immobilization of antigen and the direct electrochemistry of horseradish peroxidase (HRP) that was labeled to an antibody. A reagentless immunosensor for carcinoma antigen-125 (CA 125) determination was developed. The immunosensor was prepared by immobilizing CA 125 wit...

An immunoradiometric assay (IRMA) involving a monoclonal antibody (MAb OC125) to an ovarian carcinoma-associated antigenic determinant (CA 125) has been tested as one component in a strategy for early detection of epithelial ovarian cancer. We characterized one confirmed "false-positive" sample by murine antibody blocking studies, Western blotting, immunoaffinity, size-exclusion chromatography,...

Abstract Introduction/objective Ovarian cancer is the 6th leading cause of mortality in women, killing more women than any other reproductive system cancer. We studied expression serum micro-ribonucleic acid-21 ( miRNA-21 ) ovarian patients and explored associations with diagnosis, clinicopathological parameters, prognosis. Methods Real-time fluorescence-quantitative polymerase chain reaction w...

OBJECTIVE Colorectal cancer (CRC) is among the most common malignancies worldwide. Understanding CRC prognosis at the initial diagnosis is very important for therapeutic strategy selection. This study was conducted to evaluate the prognostic value of preoperative serum carbohydrate antigen 19-9 (CA19-9), carcinoembryonic antigen (CEA) and carbohydrate antigen 125 (CA125) for predicting 5-year r...

Background: Mesothelin is a tumor differentiation antigen; its binding to tumor-antigen CA-125 can lead to tumor metastasis. Results: Structures of the Fab from a therapeutic antibody MORAb-009 and its complex with an epitope-containing fragment of mesothelin are obtained. Conclusion: Overlapping binding sites for both CA-125 and MORAb-009 provides a basis for the antibody’s therapeutic effect....

We examined the prognostic value of normal levels of four serum cancer markers, carcinoembryonic antigen (CEA), carbohydrate associated antigen (CA19-9), alpha-fetoprotein (AFP) and cancer antigen 125 (CA125), in gastric cancer patients. Among 1927 gastric cancer patients enrolled in this study, 1477 were male (76.6%) and 450 were female (23.4%). The median age was 57 years (range 20-86). Clini...

Background The aim of this study was to evaluate the relationship between ultrasonographic findings and serum progesterone and cancer antigen-125 (CA-125) levels in threatened miscarriage and to predict pregnancy outcome. MaterialsAndMethods In a prospective comparative case-control study, serum CA-125 and progesterone levels were measured for 100 pregnant women with threatened miscarriage who ...