alstroemeria (alstroemeriaceae family) is an ornamental monocot mainly produced for its cut flowers. embryogenic calluses have been employed for gene transformation in alstroemeria. this research work was undertaken to study the embryogenic callus induction in alstroemeria cv. fuego. the vegetative explants (node, internode, leaf) along with various concentrations of auxins (picloram, naa, iaa,...

The indiscriminate use of commercial antimicrobial drugs has resulted in multiple drug resistance and adverse effects on the host. The present study was aimed to elucidate the effect of different biotic/abiotic elicitors on the growth as well as to enhance the metabolite production in leaf and epicotyl calli of Nigella sativa. Further, antibacterial activity of these callus extracts was evaluat...

BACKGROUND The threatened plant Centella asiatica L. is traditionallyused for a number of remedies. In vitro plant propagation and enhanced metabolite production of active metabolites through biotechnological approaches has gained attention in recent years. RESULTS Present study reveals that 6-benzyladenine (BA) either alone or in combination with 1-naphthalene acetic acid (NAA) supplemented ...

Purpose: Podophyllum hexandrum Royle, a source of highly valued podophyllotoxin has been subjected to heavy collection from the wild. The ever-increasing demand of podophyllum is mainly due to two semi synthetic derivatives of podophyllotoxin that is etoposide and teniposide, which are used in the treatment of various types of cancer. The anti cancer lignan derivative podophyllotoxin in Podophy...

An efficient protocol for callus induction and somatic embryogenesis of Cyclamen premium Mill. Was developed. In this study we investigate difference between response of two explants type leaf and petiole and two medium salt concentrations MS and 1⁄2 MS on callus percentage and somatic embryo number. Media was non-significant on callus production but leaf explants was better than petiole and in...

In the present study, an efficient protocol has been developed for callus induction and production of RA in callus culture of Satureja khuzistanica for the first time. In-vitro callus induction was achieved from young shoot tip explants cultured on MS and B5 media supplemented with different concentrations of IBA (0.1, 1.0, 2.0 and 5.0 mgL(-1)) solely or in combination with cytokinins BAP and K...

Astragalus species are medicinal plants which produce valuable secondary metabolites, especially cycloartane-type glycosides. In this study, stem and leaf explants of Astragalus trojanus were subjected to different plant growth regulators, environmental conditions and media compositions to identify their callus responses. Stem and leaf explants were cultured in Murashige and Skoog (MS) and wood...

since bone marrow has been shown to contain osteoprogenitor cells, an experiment was devised to test its effects when injected percutaneously into osteotomies sites in rabbit radii. in this experimental study, the osteogenicity and its effect on early bone repair of bone marrow grafts were investigated. the purpose of this study was to determine whether bone marrow grafted percutaneously led to...