Candida antarctica lipase B (CALB) is one of the most widely used and studied enzymes in the world. In order to achieve the high-level expression of CALB in Pichia, we optimized the codons of CALB gene and α-factor by using a de novo design and synthesis strategy. Through comparative analysis of a series of recombinants with different expression components, we found that the methanol-inducible ...

Abstract The synthesis of both enantiomers a key intermediate in the halofuginone was accomplished by Candida antarctica lipase B (CAL-B)-catalyzed kinetic resolution corresponding racemate. When carried out versatile solvent cyclopentyl methyl ether (CPME) using p-chlorophenylbutyrate (PCPB) as acylating reagent, highest enantiomeric ratio (E) values were measured, and highly enantioenriched (...

Lipase B from Candida antarctica (CAL-B) has been site-directedly modified by the introduction of a trans,trans-hexadiene moiety onto lipase molecules, identified by MALDI-TOF. This modification on CAL-B permitted its immobilization on Q-Sepharose supports in excellent yields (>95%) when native lipase was not immobilized at pH 7 and 25 °C. After the entire modification procedure, the catalytic ...

The N-acylation of ethanolamine (2) with various fatty acids 1a-d and esters of fatty acids 1e-h using Candida antarctica B lipase (Novozym((R)) 435) are described and optimum conditions for selective N-acylation rather than O-acylation are also discussed. Microwave assisted solution phase, solid supported and conventional methods were investigated and results were compared. There is a synergy ...

The enormous potential of nanogel scaffolds for protein encapsulation has been widely recognized. However, constructing stable polymeric nanoscale networks in a facile, mild, and controllable fashion still remains a technical challenge. Here, we present a novel nanogel formation strategy using horseradish peroxidase (HRP) catalyzed crosslinking on phenolic derivatized dendritic polyglycerol (dP...

Esterification of chitosan with adipic acid catalyzed by immobilized Candida antarctica lipase B was carried out in this study. Response surface methodology (RSM) based on a four-factor- five-level small central composite design (SCCD) was employed to model and analyze the reaction. A total of 21 experiments representing different combinations of the four reaction parameters including ch...