1. From pooled serum containing genetically homogeneous transferrin C1, transferrin was purified and separated in three fractions (tri-tri, bi-tri- and bi-bi-antennary transferrin C1), using Concanavalin A-Sepharose. 2. Each of these fractions was separated into its sialic acid-dependent subfractions by preparative isoelectric focusing. Sixteen iso-transferrin C1 fractions were obtained, which ...

Melastoma malabathricum Linn. is a perennial traditional medicine plants that grows abundantly throughout Asian countries. In this study, M. malabathricum Linn. leaf hot water crude extract with anticoagulant activity was purified through solid phase extraction cartridge and examined for the bioactive chemical constituents on blood coagulation reaction. The SPE purified fractions were, respecti...

The types of carbohydrate chains present in a rat liver lactogenic hormone-binding receptor species with an Mr of 82,000, and in its hormone-binding subunits with Mr values of 40,000 and 35,000, were characterized using carbohydrate-chain-cleaving enzymes and affinity cross-linking. The subcellular distribution of lactogenic hormone-binding species was studied in organelle-enriched fractions. T...

antiviral proteins (avp), present in silkworm fecal matter, show activity against nuclear polyhedrosis virus (npv) in vitro and in vivo. the extract of silkworm fecal matter prepared in phosphate buffer solution of ph 7.5 was subjected to 50% solid ammonium sulfate precipitation to enrich avp, then which was dialyzed. the dialysate was applied to the column containing silica gel-g, the column e...

Four wood pulps and a microcrystalline cellulose were dissolved in a NaOH 8% water solution. Insoluble fractions and clear solution fractions were isolated by centrifugation and were observed by optical microscopy and transmission electron microscopy. Molecular weight distribution, carbohydrate composition and cellulose II content were measured. The dissolution of wood cellulose fibres in NaOH ...

Avian eggshell membrane (ESM) is a natural biomaterial that has been used as an alternative natural bandage on burned and cut skin injuries for >400 years in Asian countries, and is available in large quantities from egg industries. Our aim was to characterize ESM that was separated and processed from egg waste, and to study whether this material possesses anti-inflammatory properties, making i...

Treatment of blood group substance H obtained from pig stomach linings with alkaline borohydride results in splitting off ca. 80% of carbohydrate chains. The oligosaccharide fractions obtained after removal of polypeptide were subjected to gel-, paper and anion-exchange chromatography, &29 individual reduced oligosaccharides, ranging from tnto undecasaccharide were isolated and their structures...