In mammals, transit through the epididymis, which involves the acquisition, loss and modification of proteins, is required to confer motility and fertilization competency to sperm. The overall dynamics of maturation is poorly understood, and a systems level understanding of the complex maturation process will provide valuable new information about changes occurring during epididymal transport. ...

Studies on the distribution of Na+, K+-dependent, Mg2+ dependent and Ca2+ dependent Adenosine Triphosphatase (ATP-ases) in the testes, epididymis, seminal vesicles and prostate glands of mature bonnet monkeys were carried out with and without Testosterone propionate (TP) treatment. Comparatively, the Ca2+ dependent ATP-ase was very active in the testes, caput and cauda epididymis and prostate o...

DNA stability and thiol-disulphide status of rat sperm nuclei was observed in vivo during maturation in the epididymis and penetration of oocytes. When spermatids and spermatozoa were stained with acridine orange after fixation with acetic alcohol, the red/green fluorescence ratio observed under a confocal microscope was not different between spermatids (3.81 +/- 0.16) and testicular spermatozo...

Monoclonal antibodies (mabs) have been used as a powerful tool for identification of newer sperm proteins. However, conventional hybridoma technology rarely provides chance to obtain mabs to epididymal proteins. To increase this chance, we have used an alternate method of neonatal tolerization. In this protocol, animals were tolerized at birth using testicular proteins followed by immunization ...

Micropuncture samples taken from the rete testis, caput, corpus and cauda epididymidis of adult rats were assayed for intraluminal sperm concentrations. The amount of fluid resorbed from the efferent duct and epididymal lumen was calculated based on the sperm concentration. Epididymal sperm concentrations increased from the rete fluid to the cauda fluid through the caput fluid. Eighty-nine perc...

After a serious injury or sudden death, epididymis cauda sperm recovery and cryopreservation may present as the last opportunity to obtain genetic material from a valuable stallion. This study evaluated the viability of cooled equine sperm collected by three different methods: sperm of ejaculated (G1), sperm recovered from the epididymal cauda immediately after orchiectomy (G2) and sperm recove...

Spermatozoa acquire forward motility and fertilizing capacity during their transit through the epididymis. This maturation process involves modifications of the sperm surface by different proteins secreted by a series of specialized regions in the epididymal epithelium. Previously, our lab has reported IMDS-60 gene, which is highly expressed in mouse corpus and cauda epididymidis. Here, to perf...

Utilization of cauda epididymis spermatozoa has been carried out because these can be used in artificial insemination or vitro fertilization. The purpose this study was to obtain information on the quality which produced from Bali cattle at different ages. samples came 31 slaughterhouses. Information and identification age based structure incisors. Observations were made variables motility, con...

The epididymis was reflected unilaterally or bilaterally to the abdomen in adult hamsters, leaving normally functioning testes in the scrotum. In unilateral cases, spermatozoa taken from the abdominal cauda, 1 month or more post-operatively, underwent a reversal of head agglutination and dispersed earlier, and underwent hyperactivation and fertilized cumulus-free eggs about 30-45 min sooner tha...

Some melatonin functions in mammals are exerted through MT₁ and MT₂ receptors. However, there are no reports of their presence in the reproductive tract of the ram, a seasonal species. Thus, we have investigated their existence in the ram testis, epididymis, accessory glands and ductus deferens. Real-time polymerase chain reaction (qPCR) revealed higher levels of m-RNA for both receptors in the...