نتایج جستجو برای: cbmn assay
تعداد نتایج: 221722 فیلتر نتایج به سال:
RATIONALE DNA damage and chromosomal alterations in peripheral lymphocytes parallels DNA mutations in tumor tissues. OBJECTIVE The aim of our study was to predict the presence of neoplastic colorectal lesions by specific biomarkers in "medium risk" individuals (age 50 to 75, with no personal or family of any colorectal neoplasia). METHODS AND RESULTS We designed a prospective cohort observa...
Occupational exposure to (benzene, toluene and xylene, BTX is common in the Chinese workplace. Chronic occupational exposure to benzene is associated with an increased risk of hematological malignancies such as acute myeloid leukemia (AML), but the underlying mechanisms are still unclear. This study investigates changes in poly(ADP-ribosyl)ation and DNA methylation in subjects occupationally ex...
OBJECTIVES The aim of this study is to assess chromosomal damage in Tunisian hospital workers occupationally exposed to low levels of ionizing radiation (IR). MATERIALS AND METHODS The cytokinesis-block micronucleus (CBMN) assay in the peripheral lymphocytes of 67 exposed workers compared to 43 controls matched for gender, age and smoking habits was used. The clastogenic/aneugenic effect of I...
The International Human Micronucleus (HUMN) Project (www.humn.org) was founded in 1997 to coordinate worldwide research efforts aimed at using micronucleus (MN) assays to study DNA damage in human populations. The central aims were to (i) collect databases on baseline MN frequencies and associated methodological, demographic, genetic and exposure variables, (ii) determine those variables that a...
Background: Most of the cytogenetic dosimetry, except Premature Chromosome Condensation assay, saturates at 6 Gy. Present studies are aimed to assess feasibility of simple CB-MN assay for prescreening of high dose accidental exposures. Materials and Methods: Human peripheral blood lymphocytes from two donors were gamma irradiated to different doses ranging from 0-15Gy, cultured for 72 h. Cytoch...
Estimating the effects of small doses of ionising radiation on DNA is one of the most important problems in modern biology. Different cytogenetic methods exist to analyse DNA damage; the cytokinesis-block micronucleus assay (CBMN) for human peripheral blood lymphocytes is a simple, cheap and informative cytogenetic method that can be used to detect genotoxic-related markers. With respect to pre...
Antineoplastic drugs (ANPDs) are a heterogeneous group of chemicals that include alkylating agents (e.g., cyclophosphamide, melphalan, chlorambucil), antimetabolites (e.g., thioguanine, 5-fluorouracil, methotrexate), antibiotics (e.g., doxorubicin), mitotic spindle inhibitors (e.g., vincristine), hormones (e.g., diethylstilbestrol, free radical generators (e.g., bleomycin) and topoisomerase inh...
OBJECTIVE A previous study by our research group evaluated the levels of DNA damage using the comet assay in hemodialysis patients with type 2 diabetes mellitus. The same blood samples were also evaluated using the cytochalasin B micronucleus assay. A comparison of the results of the two assays is presented here. METHODS Whole blood samples were collected from 22 type 2 diabetes mellitus pati...
Background: Sufficient epidemiologic evidence shows an etiologic link between polycyclic aromatic hydrocarbons (PAH) exposure and lung cancer risk. While the genetic modifications have been found in PAH-exposed population, it is unclear whether gene-specific methylation involves in the process of PAHassociated biologic consequence. Methods: Sixty-nine PAH-exposedworkers and 59 control subjects ...
Eleven 31-methylenlanostane triterpenoids, i.e., seven 21- and four 26-oic acids, as well a lupane triterpenoid betulin, isolated from the fruiting bodies of mushroom Fomitopsis betulina, were tested for in vitro protective effect on chromosome aberrations peripheral human lymphocytes using cytochalasin-B blocked micronucleus (CBMN) assay. Most compounds showed beneficial by reducing DNA damage...
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