Phosphatidylserine (PS) externalization is a marker for the nucleated cell apoptosis, and refers cellular activation rather than apoptosis in platelets. On the other hand, several similarities exist between platelet activation and apoptosis in nucleated cells. Herewith we investigated the relationship between platelet activation and platelet apoptosis. Platelets isolated from fresh blood of 22 ...

OBJECTIVE To construct 3 expression plasmids for the targeted therapy of thrombosis: single chain variable fragment (scFv) of monoclonal antibody (mAb) 7E3 that can identify and bind platelet glycoprotein GPIIb-IIIa complex, scFv of mAb WAPS12.2 that can identify and bind P selectin (CD62P), a diabody that can identify and bind GPIIb-IIIa and CD62P simultaneously, and to investigate whether the...

BACKGROUND AND PURPOSE Combined antiplatelet agents may offer additive protection over single drugs after stroke. We investigated whether platelet activation is reduced under combined aspirin and clopidogrel compared with each drug alone. METHODS In a case-crossover study, 31 patients with previous atherothrombotic or lacunar stroke who were treated with aspirin (100 to 300 mg/d) received clo...

Platelet activation and functional changes were investigated in acute lymphoblastic leukemia (ALL) to provide the basis for early diagnosis and evaluation of curative effect. Platelet parameters, immature platelet fraction (IPF%), immature platelet count (IPC), P-selectin (CD62p) (PAC-1) expression were detected in ALL, ALL-complete remission-induced (CR1), and normal groups with an automatic b...

Objective: As a type of arrhythmia, Atrial Fibrillation (AF) seriously affects human health. Although Radiofrequency Catheter Ablation (RFCA) has been proved to be a safe and effective method for the treatment of AF, thromboembolism caused by this treatment limited its application. Our study aimed to investigate the effects of calcium channel blocker amlodipine on thrombosis-related factors aft...

BACKGROUND Sevoflurane can be used as sedative-analgesic drug with endothelial protective properties. We tested whether low-dose sevoflurane inhalation provides sustained inhibition of detrimental granulocyte-platelet aggregation in humans. METHODS Ten healthy male volunteers were enrolled in this crossover study. Each subject inhaled sevoflurane for 1 h at 0.5-1 vol % end-tidal concentration...

BACKGROUND AND OBJECTIVE The development of prothrombotic and procoagulant states may be regulated by direct platelet-leukocyte contact mediated by membrane receptors. We have investigated the role of CD11b integrin in polymorphonuclear leukocytes (PMN) on fibrin formation and platelet reactivity with vascular subendothelium. METHODS Studies were carried out following a well-established perfu...

Background and Aims: The quality of platelets (PLT) during storage is influenced by various factors. The purpose of this study was to determine the potential effects of the air bubbles and foam in the bag of platelet and its effects on the quality of the final product. Materials and Methods: In this paired-study, the air bubbles and foam, which develops after the preparation of the platelet un...

P-selectin was shown to bind committed human hematopoietic progenitors (colony-forming unit-granulocyte-macrophage [CFU-GM] and burst-forming unit-erythroid [BFU-E]) as identified by their expression of the CD34 antigen and by in vitro clonogenic assays. In addition, P-selectin bound all precursors (pre-CFU) of committed myeloid progenitors assayed by their ability to sustain hematopoiesis in b...