In this study, the effect of light wavelength on growth rate and lipid production of Synechocystis was investigated. Continuous cultivation system was used to have uniform cell density and avoid self-shading in order to obtain more precise results. Based on previous studies, red light is more efficient than other colors in the visible spectrum for cultivation of Synechocystis; however, the opti...

Previously, we demonstrated a high quality of minerals formed by serum-free cultured jaw periosteal cells (JPCs) by Raman spectroscopy but the mineralization extent was not satisfactory. In the present study, we analyzed the proliferation and mineralization potential of human platelet lysate- (hPL-) cultured JPCs in comparison to that of FCS-cultured JPCs. By cell impedance measurements, we det...

We have developed a procedure for stepwise topographical control of network patterns and neurite connection directions between adjacent living neurons using an individual-cell-based on-chip multi-electrode array (MEA) cell cultivation system with an agarose microchamber (AMC) array. This procedure enables flexible and precise control of the cell positions and easy and flexible control of the pa...

Objective(S): The chitosan - Fe3O4 core - shell nanoparticles were synthesized. The nanoparticles should be coated properly in the shape of core-shell, so that they remain hidden from the body's immune system after coating. Effects of different molecular weight in coating were investigated. Methods: Nanoparticles coated with low and ...

Vero cells were grown adherent to microcarriers (Cytodex 1; 3 g L(-1)) using animal component free media in stirred-tank type bioreactors. Different strategies for media refreshment, daily media replacement (semi-batch), continuous media replacement (perfusion) and recirculation of media, were compared with batch cultivation. Cell densities increased using a feed strategy from 1×10(6) cells mL(...

Prolonged cultivation (>25 generations) of Saccharomyces cerevisiae in aerobic, maltose-limited chemostat cultures led to profound physiological changes. Maltose hypersensitivity was observed when cells from prolonged cultivations were suddenly exposed to excess maltose. This substrate hypersensitivity was evident from massive cell lysis and loss of viability. During prolonged cultivation at a ...

Trunks and stumps of various deciduous species act as natural habitats for Ganoderma lucidum. The chemical composition of their cell wall affects the development of fungal ligninolytic enzyme system as well as its ability to degrade lignin from the plant cell wall. Additionally, numerous compounds structurally similar to lignin can be degraded by the G. lucidum enzyme system which could take im...

Three kinds of phytoplankton were cultivated, and the contribution of dissolved organic matter (DOM) released from the phytoplankton was examined to clarify the cause of organic pollution of Lake Biwa. Microcystis aeruginosa, Staurastrum dorcidentiferum, and Cryptomonas ovata were evaluated with regard to cultivation. A significant peak (M(w): <3000 Da) was mainly detected in the algal DOM rele...

The cultivation of adult human cells of normal origin has become a routine procedure during the past several years (4). Among the first stable epithelial-like (Ep-L) cell lines derived from nor mal human tissues were those established by Chang (1954) (5) from conjunctiva, liver, kidney, and appendix. Although the HeLa strain of cells from cancer tissue had been available before this time, there...