background: in vitro model studies are becoming increasingly popular for experimental research designs. they include isolation and expansion of cells of a particular tissue, such as the nervous tissue which contributes to understanding the underlying mechanisms in many pathologies. it enables  the scrutinization of intracellular signaling pathways responsible for cell death. objectives: in the ...

background and objectives: contamination of cell lines and biological products is one of the major problems of cell culture techniques. rapid detection of mycoplasma contamination in cell culture is an important part of quality control standards in related laboratories. the aim of this study was to evaluate the efficacy of pcr in detection of myroplasma as contaminants in cell cultures and othe...

introduction: antibiotic supplements are regularly used in neuronal culture media to control contamination however, they can interfere with the neuronal excitability and affect electrophysiological properties. therefore, in this study, the effect of penicillin/streptomycin supplements on the spontaneous electrophysiological activity of hippocampal pyramidal neurons was examined. methods: electr...

background: this study was performed to induce conversion of rh strain tachyzoites of toxoplasma gondii to bradyzoites by ph changing of the cul­ture medium. methods: hela cell monolayers were infected at a 1:1 tachyzoite to cell ratio. four hours after infection, the culture medium was removed and re­placed with culture medium and 5% fcs, adjusted to ph 8 with naoh. the culture was maintained ...

microbial demulsifiers represent a potentially important product for the petroleum industry due to their specific modes of action, relative ease of preparation, biodegradability and low toxicity. an extracellular demulsifier, capable of the rapid break up of model multiple water-crude oil emulsions was obtained from the bacterial isolate ochrobactrum anthropi ripi5-1. demulsifying activity of t...

Cell growth and lactose consumption profile of five Lactobacillus Strains: bulgaricus, casei, lactis, delbrueckii and fermentum has been investigated. Experiments of cell growth and substrate utilization were conducted in batch submerged culture of whey with added lactose and some other growth factors. Fitness assessment of experimental data on the cell growth and lactose consumption by Monod k...