background: as a drug target and an antigenic agent, hiv-1 protease (hiv-1 pr) is at the center of attention for designing anti-aids inhibitors and diagnostic tests. in previous studies, the production of the recombinant protease has been faced with several difficulties; therefore, the aims of this study were the easy production, purification of the soluble form of protease in e. coli and inves...

Proteases are important industrial enzymes used in different areas of industry, mainly detergent, food and leather industries. In this study, novel alkaline protease-producing bacterium was isolated from Geinarje hot spring and examined for maximum protease activity to be utilized in washing-powder. The isolated bacterium was cultured in mineral salt medium including 2% Skim Milk. Proteolytic a...

human immunodeficiency virus type 1 (hiv-1) protease inhibitors comprise an important class of drugs used in hiv treatments. however, mutations of protease genes accelerated by low fidelity of reverse transcriptase yield drug resistant mutants of reduced affinities for the inhibitors. this problem is considered to be a serious barrier against hiv treatment for the foreseeable future. in this st...

This paper applied a simple and cost-benefit method for the production of regenerated bacterial cellulose. The inexpensive production of cellulose with complex media derived from wastewater from food industries such as molasses adds a lot of contaminants to the produced bacterial cellulose, which puts a lot of challenges in cellulose purification. Therefore, the present study aimed...

Full expression of the elastolytic phenotype of Pseudomonas aeruginosa depends on LasA, an extracellular protease with restricted specificity whose mode of action on elastin and biological role is not understood. LasA exhibits amino acid sequence homology to some bacteriolytic proteases and shares several physicochemical properties with the staphylolytic protease of P. aeruginosa. This led us t...

A large proportion of the nitrogen (N) in soil is in the form of proteinaceous material. Its breakdown requires the activity of extracellular proteases and other decomposing enzymes. The goal of our study was to better understand how carbon (C) and N availability affect soil protease activity. Several aerobic incubations were carried out with ammonium (NH4 þ) and proteins as N sources and cellu...

Several nonpeptide small molecules were designed as potential inhibitors of HIV protease and their structures were constructed by computer-aided molecular modeling and docked iwo the active site of HIV protease. Models of the complexes of inhibitors and the HIV protease were refined using nonbonded and H-bonding terms. The refined energy of selected complexes showed that the designed inhib...

The antifungal effect and mucolytic activity of a newly screened biocontrol strain Chaetomium subaffine LB-1 were researched in this study. results found that has good effects on the test plant pathogenic fungi Botrytis cinerea Pers. ex Fr., Fusarium oxysporum f. sp. cucumerinum Owen Alternaria solani (Ellis & Martin) Sorauer dual culture assay, with inhibition rates 61.39, 50.34 51.22%, respec...

hydatidosis is a zoonosis disease caused by the larva of echinococcus granulosus parasite in man and animals. the proteases enzymes are necessary for nutrition, migration and evasion of immunity of parasite into host. the aim of this study was to determine the protease activity of hydatid cyst protoscolices (psc), healthy and cystic liver tissues in order to compare of this biomarker for host a...

introduction : albumin, one of the most important plasma proteins, has a difficult process of synthesis and production. we compared two different methods for albumin purification: carboxymethyl cellulose (cm cellulose) resin exchange and diethylaminoethyl cellulose (deae cellulose) resin exchange in order to determine which resin could be more beneficial. materials and methods : two ion exchang...