Choloylglycine hydrolase (CGH, E.C. 3.5.1.24) is a conjugated bile salt hydrolase that catalyses the hydrolysis of the amide bond in conjugated bile acids. Bile salt hydrolases are expressed by gastrointestinal bacteria, and they presumably decrease the toxicity of host's conjugated bile salts. Brucella species are the causative agents of brucellosis, a disease affecting livestock and humans. C...

BACKGROUND AND OBJECTIVES The purpose of the present study was to investigate the advantages and disadvantages of verifying genetic abnormalities using array comparative genomic hybridization (a-CGH) immediately after diagnosis of congenital heart disease (CHD). METHODS Among neonates under the age of 28 days who underwent echocardiography from January 1, 2014 to April 30, 2016, neonates whos...

AIM To evaluate the role of whole genome comparative genomic hybridisation microarray (array-CGH) in detecting genomic imbalances as compared to conventional karyotype (GTG-analysis) or myeloma specific fluorescence in situ hybridisation (FISH) panel in a diagnostic setting for plasma cell dyscrasia (PCD). METHODS A myeloma-specific interphase FISH (i-FISH) panel was carried out on CD138 PC-e...

Comparative genomic hybridization (CGH) is an alternative molecular cytogenetic technique that can characterize unbalanced and often unrecognizable G-banded cytogenetic material in a one-step global screening procedure. It has the distinct advantage of providing a genome wide search without any prior information of the chromosomal aberration in question. CGH analysis provides information on the...

Background: Among chromosomal deletions and gains that are prognostically significant in chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), unfavorable 17p deletions are the strongest predictor of outcome. Genetic abnormalities with lesser prognostic significance include del 11q 22.3, trisomy 12, del 13q14, and an additional locus as chromosome 13q34, all of which are routinely ...

We report the use of comparative genomic hybridisation (CGH) to define the origin of a supernumerary ring chromosome which conventional cytogenetic banding and fluorescence in situ hybridisation (FISH) methods had failed to identify. Targeted FISH using whole chromosome 19 library arm and site specific probes then confirmed the CGH results. This study shows the feasibility of using CGH for the ...

A method for reducing errors in aspherical mirror testing using a computer-generated hologram (CGH) is described. By using a modified filtering method the carrier frequency in the CGH can be reduced by twothirds, and the resulting error due to distortion is only one-half of that of a conventional CGH. By adopting a Fizeau-type optical setup, only the surface quality of the reference affects the...

The aim of this study is to determine if the use of preimplantation genetic screening (PGS) by array comparative genomic hybridization (array CGH) and transfer of a single euploid blastocyst in patients with repeated implantation failure (RIF) can improve clinical results. Three patient groups are compared: 43 couples with RIF for whom embryos were selected by array CGH (group RIF-PGS), 33 coup...

Computer-generated hologram (CGH) null correctors are used as accuracy standards for interferometric measurements of optical surfaces and systems. Diffractive optics calibrators (DOCs) have been developed to evaluate the phase tolerance CGHs based on scalar approximation by measuring variations in duty cycle etching depth. However, if grating period a CGH < 5λ, is not accurate analysis recon...

Assessing variations in DNA copy number is crucial for understanding constitutional or somatic diseases, particularly cancers. The recently developed array-CGH (comparative genomic hybridization) technology allows this to be investigated at the genomic level. We report the availability of a web tool for analysing array-CGH data. CAPweb (CGH array Analysis Platform on the Web) is intended as a u...