BACKGROUND The GA2LEN chamber has been developed as a novel mobile allergen exposure chamber (AEC) allowing standardized multicenter trials in allergy. Hitherto, subjective nasal symptom scores have been the most often used outcome parameter, but in standardized modern trials objective parameters are preferred. Despite its practicability, the objective parameter peak nasal inspiratory flow (PNI...

background: allergy is a clinical disorder affecting the human population with wide geographical distribution. platanus orientalis (p. orientalis) trees are planted in many countries and their pollen causes allergic reactions. cyclophilin has recently been identified as one of the most important allergens of p. orientalis pollen. we aimed to clone and purify this allergen in escherichia coli fo...

Epicutaneous allergen-specific immunotherapy (EPIT) is proposed as an alternative route for allergen-specific immunotherapy (AIT). The induction of allergen-specific blocking IgG antibodies represents an important mechanism underlying AIT, but has not been investigated for EPIT. Here, we compared the induction of allergen-specific blocking IgG in outbred guinea pigs which had been immunized wit...

BACKGROUND Bronchial challenge with allergen causes a specific form of airways inflammation consisting of an influx of neutrophils, eosinophils, and T cells. Because the relevance of the challenge model to clinical asthma is uncertain, the cellular changes that occur in the lungs of asthmatic subjects during natural seasonal allergen exposure were investigated. METHODS Seventeen grass pollen ...

molecular evidence on the heterogeneity present in the ole e 1 allergen of the olive pollen is emerging. such polymorphism is dependent on the cultivar origin of pollen, which also determines wide differences in the expression of this protein. determination of biochemical and molecular characteristics of ole e 1 pollen allergen in two iranian olive cultivars, namely 'rowghani' and 'zard' is nec...

BACKGROUND Allergen immunotherapy (AIT) is the practice of administering gradually increasing quantities of an allergen extract to an allergic subject to ameliorate the symptoms associated with the subsequent exposure to the causative allergen. It is the only treatment that may alter the natural course of allergic diseases. According to AIT guidelines and summary of product characteristics (SmP...

during past few years, the cupressus arizonica has been abundantly planted in tehran, causing a significant increase of allergic diseases from the middle of winter to the beginning of spring. the aim of this study was the comparison of pollen protein content in two major varieties of c. arizonica planted in tehran, including c. arizonica var. arizonica and c. arizonica var. glabra , in order to...

Summary Different phenotypes of allergic rhinitis have been identified based on the seasonality of the allergen involved. Within pollinosis, importance has to be paid to the responsible pollen species. Guidelines for clinical management are mostly based on studies performed in patients with grass pollen allergy. Only few data is available on tree pollen allergy and more specifically on cypress ...

BACKGROUND We have developed a recombinant B cell epitope-based vaccine (BM32) for allergen-specific immunotherapy (AIT) of grass pollen allergy. The vaccine contains recombinant fusion proteins consisting of allergen-derived peptides and the hepatitis B surface protein domain preS as immunological carrier. METHODS We conducted a randomized, double-blind, placebo-controlled AIT study to deter...

Background: The incidence of allergic and asthmatic diseases has been continuously increased in both industrial and developing countries. Extracts from various known allergens are used for the diagnostic and therapeutic purposes. Objective: To investigate the effects of an extract prepared from Chenopodium album (Ch.A.) pollen to induce allergic asthma in BALB/C mice. Methods: BALB/C mice were ...