objective(s): this study aimed to find out whether the chick cardiomyocyte micromass (mm) system could be employed to predict the teratogenecity of common environmental factors. different multivitamins and over the counter drugs were used in this study.   materials and methods: white leghorn 5-day-old embryo hearts were dissected and trypsinized to produce a cardiomyocyte cell suspension in dul...

The effects of dibutyryl cyclic 3',5'-adenosine monophosphate (BcAMP) and Sephadex G-25 fractions of chick embryo extract on the growth rate, morphology, and pigmentation of normal chick retinal pigmented epithelium (PE) were investigated . Seven cloned PE cell lines were each grown in modified Ham's F-12 medium alone (F-12), or in F-12 supplemented with either high molecular weight (H) or low ...

Primary and passaged cultures of chick embryo fibroblasts, prepared from 10-day embryonic skin and 12-day embryonic skeletal muscle, synthesize and secrete hyaluronidase at level comparable to the tissues of origin. The level of cell-associated hyaluronidase remains approximately constant during culture while the enzyme accumulates extracellularly such that within a 48-h incubation period, leve...

The several and sometimes paradoxical effects of cortisone and its analogues on the course of a variety of infections may be duplicated in the relatively simple chick embryo-influenza virus model in which the interrelationships of a single obligate intracellular parasite and the cells of a single target organ (the allantoic entoderm) may be examined. Even in this simple system the influence of ...

T his issue of the Journal marks the fiftieth anniversary of the paper where Keith Porter, Albert Claude, and Ernest FuUam published the first electron micrograph of a complete cell (1). The cell was a fibroblast grown in tissue culture from the foregut of a chick embryo. The March 1945 article in which this micrograph appears, "A Study of Tissue Culture Cells by Electron Microscopy" opened the...

The chick embryo is a valuable tool in the study of early embryonic development. Its transparency, accessibility and ease of manipulation, make it an ideal tool for studying the formation and initial patterning of the nervous system. This video demonstrates how to graft organizer tissue into a host, a method by which Hensen s node (the organizer in the chick embryo) is grafted to a host compete...

T his issue of the Journal marks the fiftieth anniversary of the paper where Keith Porter, Albert Claude, and Ernest FuUam published the first electron micrograph of a complete cell (1). The cell was a fibroblast grown in tissue culture from the foregut of a chick embryo. The March 1945 article in which this micrograph appears, "A Study of Tissue Culture Cells by Electron Microscopy" opened the...

This study evaluates the suitability of flow cytometry with the fluorochrome BCECF for measuring the intracellular pH (pHi) of cultured cells, and monitors the changes in pHi in murine hybridoma in batch culture and chick embryo fibroblast in monolayer culture (5th passage). The technique produced highly reproducible, repeatable results. The theoretical sensitivity from the calibration curve wa...

Electromagnetically induced currents pulsating at very low frequency stimulated calcium uptake by chick embryo tibia rudiments only when bicarbonate was present in the culture medium. A bicarbonate-dependent Ca2+-ATPase might be implicated in coupling of the electromagnetic signal with processes that promote Ca-transport and storage in bone tissue.