The effect of orally fed Maharishi Amrit Kalash was examined on the activities of cholinergic enzymes in the guinea pig brain. The activity of the cholinergic enzymes viz. choline-acetyltransferase and acetylcholinesterase enzymes was found to be reduced significantly (P<0.05) in the various regions of CNS of the aged guinea pigs. Oral administration of MAK(500 mg/kg body weight daily) for 2 mo...

Choline acetyltransferase (EC 2.3.1.6) was studied in human cerebrospinal fluid and blood. Sensitivity, precision and reproducibility of the radioactive assay were evaluated with a homogenate from pig brain and with partially purified enzymes from bovine brain and human placenta. Contrary to other reports, with the assay used in the present study, choline acetyltransferase activity was not dete...

Various anatomically distinct regions from postmortem human brain were analyzed for o-bungarotoxin (a-BuTX) binding and choline acetyltransferase activity. There was a heterogeneous distribution of both cr-BuTX and enzyme activity in the regions studied. Although levels of cY-BuTX binding were lower than those reported in other mammalian brain studies, the relative regional concentration was, i...

Diabetes often correlates with tau phosphorylation and the development of Alzheimer's disease. Both are associated with brain cholinergic dysfunction that could benefit from nerve growth factor (NGF)-based therapies. Electroacupuncture (EA) improves brain NGF availability and action. Here we assessed the variations of NGF and tau phosphorylation in the cortex and hippocampus, as well as the exp...

To examine the possible cholinergic nature of the Mauthner cell, freeze-dried sections of goldfish spinal cord were prepared, enabling access to the Mauthner axons. Profiles of the Mauthner axons were dissected out, weighed, and quantitatively assayed for activity of choline acetyltransferase. The Mauthner axon was shown to contain significantly less activity than surrounding tissue containing ...

Sequential immunohistochemical demonstration of nerve growth factor receptor and cholinergic acetyltransferase on the same tissue section in the rat revealed that approximately 92% of all cholinergic neurons in the basal forebrain possessed that receptor. Only 0.9% of the neurons demonstrating nerve growth factor receptor in the basal nuclear complex lacked the cholinergic synthetic enzyme, and...

Xenopus oocytes were injected with poly(A)+ mRNA obtained from the electric lobes of Torpedo marmorata and Torpedo ocellata, which contain the cell bodies of the neurons that innervate the electric organs. The electric lobe mRNA preparation induces the oocytes to synthesize a catalytically active form of the enzyme choline acetyltransferase (EC 2.3.1.6). Enzymatic activity is found almost exclu...

Ahstract Chi>line ;icctyltransfcrase (EC' 2.3.1.6) catallw, the following reversible rcaction: acetyl coen/jmc A + choline e acctylcholine + cocn7yme A. Enzyme activity in the atria and ventricles of guineapig h u r t wried indcpendcntly of the biochemically rclatcd carnitine acetyltransferasc (EC 2.3.1.7). Choline acctyltransfcrasc activity was greatest in right atrium. intermediate in right v...