The use of liquid chromatography-mass spectrometry (LC-MS) has recently exploded in various analytic fields, including toxicology and therapeutic drug monitoring (although still far behind pharmacokinetics). There is no doubt that LC-MS is currently competing with gas chromatography (GC)-MS for the status of the reference analytic technique in toxicology. This review presents, for the nonspecia...

The cross-sensitivity of breath-alcohol analysing systems is intensively discussed since these instruments are used for evidential breath testing. Besides ethanol other substances can be present in human breath at significant concentrations. In the beginning of the design of such instruments most of the interest was concentrated only on aceton, which can be found in the breath of subjects, who ...

Instrumental bitterness assessment of traditional Chinese herbal medicine (TCM) preparations was addressed in this study. Three different approaches were evaluated, high-performance liquid chromatography coupled to UV detector (HPLC), capillary electrophoresis coupled to UV detector (CE) and a potentiometric multisensor system - electronic tongue (ET). Most studies involving HPLC and CE separat...

Liquid chromatography (LC) coupled to mass spectrometry (MS) or tandem mass spectrometry (MS/MS) has become increasingly important in clinical and forensic toxicology as well as doping control and is now a robust and reliable technique for routine analysis in these fields. In recent years, methods for LC-MS(/MS)-based systematic toxicological analysis using triple quadrupole or ion trap instrum...

We studied the effect in vitro and in vivo of dipyrone on the determination of several biochemical tests in two analyzers, a Hitachi 747 and a Kodak Ektachem 700. From studies in vitro, we found significant interference by dipyrone (P < 0.05) in the determination of creatine kinase (CK), lactate dehydrogenase (LD), uric acid, triglycerides, cholesterol, aspartate aminotransferase, alanine amino...

The atmosphere simulation chamber SAPHIR at the Research Centre Jülich was used to test the suitability of state-of-the-art analytical instruments for the measurement of gas-phase formaldehyde (HCHO) in air. Five analyzers based on four different sensing principles were deployed: a differential optical absorption spectrometer (DOAS), cartridges for 2,4-dinitrophenylhydrazine (DNPH) derivatizati...

Liquid chromatography, coupled with tandem mass spectrometry, has become one of the most popular methods for the analysis of post-transcriptionally modified transfer ribonucleic acids (tRNAs). Given that the information collected using this platform is entirely determined by the mass of the analyte, it has proven to be the gold standard for accurately assigning nucleobases to the sequence. For ...

phytosterols have been used for reduction of cholesterol since 1950. the high melting temperature and chalky taste are the major problems related to the enrichment of foodstuffs with phytosterols. the cristal construct of these compounds could not reduce remarkably the blood cholesterol in comparison with the fatty soluble forms. presence of free and alcoholic forms of phytosterols and also the...

This review enlightens the role of supercritical fluid chromatography (SFC) in the field of lipid analysis. SFC has been popular in the late 1980s and 1990s before almost disappearing due to the commercial success of liquid chromatography (LC). It is only 20 years later that a regain of interest appeared when new commercial instruments were introduced. As SFC is fully compatible with the inject...

General. 1H and C NMR spectra were measured on Varian Gemini 2000 (300 MHz for H) instruments, and are reported in parts per million (δ) from internal tetramethylsilane or residual solvent peak. IR spectra were recorded on a Shimadzu FTIR-8200, and absorptions are reported in cm. FAB-MS spectra were recorded on JEOL IMS-300 spectrometer. 3-Nitrobenzyl alcohol was used as a matrix for FAB-MS. Me...