BACKGROUND Failed fertilization occurs in 2-3% of ICSI cycles and is mainly due to lack of oocyte activation. Heterologous ICSI of patient's sperm in mouse oocytes allows discrimination between sperm- and oocyte-related aetiologies of activation failure. Assisted oocyte activation (AOA) by Ca-ionophore treatment can initiate fertilization in subsequent therapeutic ICSI. We report on diagnosis a...

We review the study of flower color polymorphisms in the morning glory as a model for the analysis of adaptation. The pathway involved in the determination of flower color phenotype is traced from the molecular and genetic levels to the phenotypic level. Many of the genes that determine the enzymatic components of flavonoid biosynthesis are redundant, but, despite this complexity, it is possibl...

background: echinococcus granulosus cultivation is very important for improvement of different aspect of medical and veterinary researches. despite many advances in this case, there is a missing link for in vitro life cycle of adult worms and it is fertili-zation. regarding the researchers’ observations, self-fertilization can be done in worms living in dog intestine, but despite all sorts of e...

the success rate of several advanced basic and clinical techniques in the field of mammalian biotechnology, including cloning, pre-implantation genetic diagnosis, and assisted reproductive techniques (art) depends mainly on the success rate of pregnancy following in vitro fertilization-embryo transfer (ivfet). the techniques used in art have advanced considerably since the first in vitro fertil...

OBJECTIVE Determine whether testicular sperm extractions and pregnancy outcomes are influenced by male and female infertility diagnoses, location of surgical center and time to cryopreservation. PATIENTS One hundred and thirty men undergoing testicular sperm extraction and 76 couples undergoing 123 in vitro fertilization cycles with testicular sperm. OUTCOME MEASURES Successful sperm recove...

Objective: To investigate the effect of sperm DNA fragmentation index (DFI) on the clinical outcomes of in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). Methods: Infertile couples were prospectively recruited in the Reproductive Center in Jiaozuo Maternal and Child Care Service Centre in Henan, China, from July 2015 to December 2016. All the recruited 228 infertile coup...

BACKGROUND Generally, intracytoplasmic sperm injection (ICSI) may be the preferable method to treat partial globozoospermia, but whether there exist some correlations between ICSI fertilization rate and the proportion of round-headed sperm or morphologically normal sperm remains open. This study was to explore the correlation between ICSI fertilization rate and the sperm morphology in patients ...

background: conventional ivf and icsi are two common techniques to achieve fertilization. ivf has long been used for treatment of infertility, although it is not an effective treatment in severe male infertility. the use of icsi has been expanded in severe male factor and fertilization failure after ivf cycle. in spite of the widespread use of icsi in patients with non-male factor infertility, ...

Background: Chemical activation is the most frequently method for artificial oocyte activation, resultant in high fertilization rates. The aim of this prospective randomized controlled study is evaluating the effect of calcium ionophore on fertilization and pregnancy rate in infertile men suffering from teratoospermia. Materials and Methods: 38 women with teratoospermic partner were divided int...