background and objectives: we developed and evaluated the utility of a quadruplex taqman real-time pcr assay that allows simultaneous identification of vancomycin-resistant genotypes and clinically relevant enterococci. materials and methods: the specificity of the assay was tested using reference strains of vancomycin-resistant and susceptible enterococci. in total, 193 clinical isolates were ...

Objective: to evaluate the morphological changes in diabetic foot syndrome emphasizing ultrastructural cellular changes.Material and methods. Biopsies of chronic lesions 120 patients following necessary preparatory stages were studied by histological, histochemical electron microscopy methods.Results. The inflammatory infiltration destruction involving all layers skin soft tissue, phenomena vac...

Background: Staphylococcus hominis has been recently emerged as an opportunistic hospital-associated pathogen. The aim of this study was to characterize the antimicrobial susceptibility pattern, biofilm formation, and staphylococcal cassette chromosome mec (SCCmec) types among clinical isolates of S. hominis obtained from pediatric infections. Methods: Totall...

Recent outbreaks of nosocomial infections caused by organisms identified as the Mycobacterium fortuitum complex suggest that species and subspecies identification is epidemiologically important. In a study of 170 strains, M. fortuitum was differentiated from M. chelonei by nitrate reduction and iron uptake. M. fortuitum was further divided into biovariant fortuitum, biovar peregrinum, and an un...