نتایج جستجو برای: clone purification
تعداد نتایج: 94232 فیلتر نتایج به سال:
Improved methods for growing animal cells in culture have led to their wide use as a tool in a variety of disciplines in biology. Cultures from many different anatomical origins are available and purification of several of them has been effected by the method of single clone isolation (1). The exact nature of the cultured cells and their relationship to the organ of their origin is, however, un...
............................................................................................................................. ii Acknowledgements .......................................................................................................... iv List of Figures .................................................................................................................. vii List o...
Biochemical and biosynthetic evidence has been obtained which indicates that the macrophage activating factor (MAF) produced by the murine T cell hybridoma clone 24/G1, which primes macrophages for nonspecific tumoricidal activity, is a form of gamma-interferon (IFN gamma). MAF and antiviral activities were generated by the clone in proportional amounts under a variety of culture conditions. Pr...
BACKGROUND Expression and purification of correctly folded proteins typically require screening of different parameters such as protein variants, solubility enhancing tags or expression hosts. Parallel vector series that cover all variations are available, but not without compromise. We have established a fast, efficient and absolutely background free cloning approach that can be applied to any...
A chimeric yellow fever-dengue 1 (ChimeriVax-DEN1) virus was produced by the transfection of Vero cells with chimeric in vitro RNA transcripts. The cell culture supernatant was subjected to plaque purification for the identification of a vaccine candidate without mutations. Of 10 plaque-purified clones, 1 containing no mutation (clone J) was selected for production of the vaccine virus. During ...
CP5 system, for simple and highly efficient protein purification with a C-terminal designed mini tag
There are many strategies to purify recombinant proteins of interest, and affinity purification utilizing monoclonal antibody that targets a linear epitope sequence is one of the essential techniques used in current biochemistry and structural biology. Here we introduce a new protein purification system using a very short CP5 tag. First, we selected anti-dopamine receptor D1 (DRD1) rabbit monoc...
background and objectives: phytoplanktons are organisms with a very high diversities and global distribution in different habitats. the high distribution of phytoplankton is due to ecological flexibility and their ability to tolerate different climatic conditions and environmental stress. phytoplankton is the most sensitive biological indicators of water resources. the purpose of this study was...
the development of a therapeutic vaccine against human papillomavirus (hpv) is important for the control of cervical cancer. e7 is the major transforming protein produced in cervical cancers, and therefore represents potential tumor-specific antigen that could be the target of immunotherapy for cervical cancer. among different vaccine strategies, protein-based vaccines are capable of generating...
نمودار تعداد نتایج جستجو در هر سال
با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید