In conventional quantum mechanics, no-deleting and no-cloning theorems indicate that two different nonorthogonal states cannot be perfectly deterministically deleted cloned, respectively. Here, we investigate the deleting cloning in a pseudo-unitary system. We first present pseudo-Hermitian Hamiltonian with real eigenvalues two-qubit By using operators generated from this Hamiltonian, show it i...

The accessory cell requirements for cloning T cells in the presence of lectin and T cell growth factors were examined with cells from human peripheral blood. We found that dendritic cells were active and perhaps essential. Single CD4+ lymphocytes could be cloned with 80% efficiency, and CD8+ cells with 50-60% efficiency if 10(3) syngeneic or allogeneic dendritic cells were added. Some T cell cl...

PCR fragments and linear vectors containing overlapping ends are easily assembled into a propagative plasmid by homologous recombination in Escherichia coli. Although this gap-repair cloning approach is straightforward, its existence is virtually unknown to most molecular biologists. To popularize this method, we tested critical parameters influencing the efficiency of PCR fragments cloning int...

A network of agents constituted multiple unmanned aerial vehicles (UAVs) is emerging as a promising technology with myriad applications in the military, public, and civil domains. UAVs’ power, memory, size constraints, ultra-mobile nature, non-trusted operational environments make them susceptible to various attacks, including physical capturing cloning attacks. robust resilient security protoc...

Herein, we describe a novel cloning strategy for PCR-amplified DNA which employs the type IIs restriction endonuclease BsaI to create a linearized vector with four base-long 5'-overhangs, and T4 DNA polymerase treatment of the insert in presence of a single dNTP to create vector-compatible four base-long overhangs. Notably, the insert preparation does not require any restriction enzyme treatmen...

Probabilistic cloning was first proposed by Duan and Guo. Then Pati established a novel cloning machine (NCM) for copying quantum superposition of multiple clones. In this paper, we deal with the novel cloning machine with supplementary information (NCMSI) for obtaining quantum superposition of multiple clones. For the case of cloning two states, we demonstrate that the optimal efficiency of th...

Probabilistic cloning was first proposed by Duan and Guo. Then Pati established a novel cloning machine (NCM) for copying superposition of multiple clones simultaneously. In this paper, we deal with the novel cloning machine with supplementary information (NCMSI). For the case of cloning two states, we demonstrate that the optimal efficiency of the NCMSI in which the original party and the supp...

A robust method for the in vivo cloning of large gene clusters was developed based on homologous recombination (HR), requiring only the transformation of PCR products into Escherichia coli cells harboring a receiver plasmid. Positive clones were selected by an acquired antibiotic resistance, which was activated by the recruitment of a short ribosome-binding site plus start codon sequence from t...