A soft agarose clonogenic assay is presented which has been optimized for the growth of human gastrointestinal adenocarcinomas. Samples from 15 gastric and colonic solid tumors and from 2 noncancerous stomachs (control cultures) were disaggregated by treatment with collagenase at 37 degrees overnight. Colonies appeared 10 to 15 days after plating, with a cloning efficiency between 0 and 0.82%, ...

Piperine is a dietary alkaloid with huge pharmacological importance and was first isolated from pepper extract. It has been reported anti-cancer, anti-amoebic, anti-mycobacterial, anti-mutagenic, anticonvulsant, anti-asthmatic, immunomodulatory, anti-inflammatory anti-oxidant activity. Therefore, the current investigation designed to unveil anticancer effects of piperine molecule against human ...

The anticancer drug sensitivity of human cancers was tested by the human tumor clonogenic assay (HTCA). Of 152 human cancer specimens tested, 63 (41%) formed more than 30 tumor cell colonies in control plates and could be used to evaluate the drug sensitivity of tumor cells. In 42 (93%) of 45 clinical trials in 24 patients, a parallel correlation was observed between the in vitro anticancer dru...

Two culture methods are available for the study of the blast cells of acute myeloblastic leukemia (AML). One is an assay for clonogenic precursors; it depends on their ability to form blast colonies in culture in the presence of methylcellulose and suitable growth factors. The other assesses the growth of blast cells in suspension culture, where growth is measured by increasing numbers of clono...

Abstract Diffuse intrinsic pontine glioma (DIPG) is a universally fatal childhood cancer of the brain with 5-year survival rates < 2%. Despite introduction conventional chemotherapy and radiotherapy, improvements in have been marginal long-term survivorship rare. We previously developed MP-Pt(IV), monoamine oxidase B (MAOB) sensitive, mitochondrial targeting platinum-(IV) based prodrug d...

Background: Radiation-induced lung injury (RILI) is a serious concern in carbon-ion radiation therapy (CIRT) for thoracic malignancies. To estimate the induction of RILI after CIRT, translation of evidence in X-ray radiation therapy to CIRT is of great importance. However, the relative biological effectiveness (RBE) of carbon-ion beams in normal lung tissues is not fully elucidated, making the ...

The aim of the present study was to evaluate cytotoxic potential a new complex silver(I) with furosemide (Ag-FSE) over panel tumor and non-tumor human cells, as well its genotoxicological safety. According results obtained, cell line derived from colorectal adenocarcinoma (Caco-2) most sensitive Ag-FSE complex, among cells non-transforming fibroblasts known GM07492A, which stimulated characteri...

To develop a purification strategy for isolating the most primitive hematopoietic stem cells present in normal human marrow we have combined cell separation techniques with an assay for cells that initiate sustained hematopoiesis in vitro in the presence of irradiated human marrow adherent cells. These "feeders" were established by subculturing 2- to 6-week-old primary long-term marrow culture ...

Aesculetin is an active member of coumarins that has been reported to possess significant medicinal and biological importance. It also shown with potential anticancer activity against different human cancers including breast, lung hepatocellular carcinoma. Therefore, the current investigation was undertaken examine effects aesculetin gastric cancer. MTT assay performed check cellular viability ...