The crucian carp Carassius carassius (Linnaeus, 1758), is native to many European freshwaters. Despite its wide distribution, the crucian carp is declining in both the number and sizes of populations across much of its range. Here we studied 30 individuals of a putative pure population from Helsinki, Finland. Despite clear external morphological features of C. carassius, an individual was of a ...

introduction: pronuclear size, morphology and orientation are known to affect embryo quality and implantation. the present study was aimed at evaluation of the possible relationship between protamine deficiency with pronuclear morphology and also cleavage and embryo quality. material and methods: semen sample of 160 candidate icsi and ivf couples were analyzed for sperm concentration, and motil...

introduction: the objective of this study was to evaluate the relationship between spermiogenic defects such as protamine deficiency, and acrosomal integrity with fertilization and pregnancy rate in ivf patients. material and methods: density, motility and morpology of semen samples from 70 infertile couples undergoing ivf at isfahan fertility and infertility center were analyzed according to w...

background: globozoospermia is a severe form of teratozoospermia (incidence < 0.1%) in infertile men that is characterized by round headed sperm and acrosomeless in semen. objective: to compare the semen parameters, protamine deficiency, and apoptosis in ejaculated spermatozoa between globozoospermic and normozoospermic men. materials and methods: thirty six semen samples were divided into two ...

Background: Current microscopic experimental methods cannot diagnose DNA damages present in spermatozoa .Therefore, some methods are needed to address the abnormality of the genetic material status on the sperm samples. As reported by many investigators aniline blue staining technique has been used for identifying sperm chromatin condensation. Also, chromomycin A3 is used for evaluation of the ...

Background The objective of this study was to evaluate the relationship between protamine deficiency, and acrosomal integrity with fertilization and pregnancy rate in patients undergone in vitro fertilization (IVF). MaterialsAndMethods Semen samples from 70 infertile couples undergoing IVF at Isfahan Fertility and Infertility center were assessed in this study. Semen analysis was carried out ac...

Background: This study investigated the probable effects of daily administration of methamphetamine (MA) on sperm motility, acrosome reaction (AR), acridine orange (AO), sperm chromatin dispersion (SCD) test and chromomycin A3 (CMA3) in mice. Materials and Methods: Twenty four NMRI male mice were administrated three different doses of MA intraperitoneally (n=6 in each group). Different doses of...

Infertility is considered as one of the major problems associated with spinal cord injury (SCI). However, the exact underlying mechanism is still unknown. Therefore, the main objective of this experimental study was to evaluate the effect of chronic SCI on sperm parameters as well as chromatin integrity and DNA of spermatozoa aspirated from cauda epididymis of rats. Forty-five adult Wistar rats...

In this study, the description of the karyotypes of the endangered chubs Squalius aradensis (Coelho, Bogutskaya, Rodrigues and Collares-Pereira, 1998) and Squalius torgalensis (Coelho, Bogutskaya, Rodrigues and Collares-Pereira, 1998) is presented by means of conventional (Giemsa-staining, Chromomycin A3 (CMA3)-fluorescence, Silver-impregnation (Ag-NORs)) and molecular (fluorescence in situ hyb...

The Tetraodontiformes are the most derived group of teleostean fish. Among other apomorphies, they are characterized by a high degree of fusions or significant bone loss in the head and body. In the early phylogenetic proposals presented for this order, the families Balistidae and Monacanthidae have been unanimously considered to be closely related. Although they have moderate species diversity...