Nine isolates of cellulose-degrading Actinomycetes were isolated from different sediment samples from the Bhitarkanika Mangrove Forest. Cellulase and hydrolytic activity was confirmed with clear zone around the colony on basal salt agar medium using Congo Red. The enzyme assays for two enzymes, filter paper cellulase (FPCase), and carboxymethylcellulase (CMCase), were examined by methods recomm...

Cellulolytic enzymes produced by spore-forming bacteria seem to be a potential solution the degradation of lignocellulosic waste. In this study, several dozen bacterial strains were isolated from soil and one them was selected for further studies. The studied strain identified genus Bacillus (strain 8E1A) 16S rRNA gene sequencing. sp. 8E1A showed an activity carboxymethyl cellulase (CMCase) wit...

A Bacillus isolate AK5 which was provisionally identified as Bacillus brevis isolated from the Acacia forest soil at University of Chittagong was found to be capable of producing cellulolytic enzymes during growth on different cellulosic substrates. The isolate AK5 grown under different conditions showed that CMC was the best cellulosic substrates for inducing the synthesis of extracellular cel...

A thermophilic strain AMF-07, hydrolyzing carboxymethylcellulose (CMC) was isolated from Kerman hot spring and was identified as Bacillus licheniformis based on 16S rRNA sequence homology. The carboxymethylcellulase (CMCase) enzyme produced by the B. licheniformis was purified by (NH4)2SO4 precipitation, ion exchange and gel filtration chromatography. The purified enzyme gave a single band on S...

The present work deals with the development of solid state fermenter for enzymes production and the development of a pilot scale of enzymatic deinking system. The newly developed solid state fermenter, was named FERMSOSTAT®, which was used for the production of cellulases and xylanase using a local isolate; Aspergillus niger USM AI 1. Optimization of enzymes production was carried out and the o...

Bacteria isolated from thermophilic environment that can produce cellulase as well as utilise agro-waste biomass have a high potential for developing thermostable cellulase required in the biofuel industry. The cost for cellulase represents a significant challenge in converting lignocellulose to fermentable sugars for biofuel production. Among three potential bacteria examined, Bacillus licheni...

Effect of physical parameters such as initial pH, agitation (rpm), and temperature (°C) for cellulase production from Bacillus subtilis AS3 was investigated. Central composite design of experiments followed by multiple desirability function was applied for the optimization of cellulase activity and cell growth. The effect of the temperature and agitation was found to be significant among the th...

Aspergillus niger was used for cellulase production in submerged (SmF) and solid state fermentation (SSF). The maximum production of cellulase was obtained after 72 h of incubation in SSF and 96 h in Smf. The CMCase and FPase activities recorded in SSF were 8.89 and 3.56 U per g of dry mycelial bran (DBM), respectively. Where as in Smf the CMase & FPase activities were found to be 3.29 and 2.3 ...

Seven agricultural wastes named wheat bran (WB), rice straw (RS), wheat straw (WS), corn cob (CC), sugarcane bagasse (SCB), potato peal (PP) and banana peal (BP) were used for isolation of bacterial strains and as substrate for cellulases (CMCase, FPase and avicelase) and xylanase production. Five of the isolated strains were thermophilic and alkaliphilic. These bacterial isolates were tested t...

Extreme habitats have usually been regarded as a source of microorganisms that possess robust proteins that help enable them to survive in such harsh conditions. The deep sea can be considered an extreme habitat due to low temperatures (<5°C) and high pressure, however marine sponges survive in these habitats. While bacteria derived from deep-sea marine sponges have been studied, much less info...