1. Incubation of cell-free extracts of methane- or methanol-grown Pseudomonas methanica with [(14)C]formaldehyde and d-ribose 5-phosphate leads to incorporation of radioactivity into a non-volatile product, which has the chromatographic properties of a phosphorylated compound. 2. Treatment of this reaction product with a phosphatase, followed by chromatography, shows the presence of two compoun...

Mitotic chromosome condensation is a prerequisite for the accurate segregation of chromosomes during cell division, and the conserved condensin complex a central player of this process. However, how condensin binds chromatin and shapes mitotic chromosomes remain poorly understood. Recent genome-wide binding studies showing that in most species condensin is enriched near highly expressed genes s...

We examined whether the well-known neurotoxins 6-hydroxydopamine (6-OHDA) and 1-methyl-4-phenylpyridinium ion (MPP(+)) recruit distinct cell death mechanisms using primary cultured neurons derived from day 16 embryonic rat cortices. Electron microscopy revealed that cell death induced by both 6-OHDA and MPP(+) was typified by a condensation of chromatin while prominent mitochondrial swelling wa...

Herein we report the synthesis of tripodal C3-symmetric opioid scaffolds as high-affinity condensation agents of duplex DNA. Condensation was achieved on both supercoiled and canonical B-DNA structures and identified by agarose electrophoresis, viscosity, turbidity and atomic force microscopy (AFM) measurements. Structurally, the requirement of a tris-opioid scaffold for condensation is demonst...

We report the formation of Au/Co nanoparticles and their characterization by aberration (Cs) corrected scanning transmission electron microscopy (STEM). The nanoparticles were synthesized by inert gas condensation, forming initially core-shell and bimetallic crystals. However, after thermal treatment at normal atmospheric conditions, the Co nanoparticles changed their morphology into a fine lay...

We present single-dish and interferometric observations of gas and dust in the core of the Serpens molecular cloud, focusing on the northwestern condensation. Single-dish molecular line observations are used to probe the structure and chemistry of the condensation while high-resolution images of CS and CH 30H are combined with continuum observations from A. = 1.3 mm to A. = 3.5 em to study the ...

Macrocyclic complexes of Mn, Co, Ni and Cu using a macrocyclic ligand derived by the condensation of Trimethoprim and diethyl phthalate have been prepared and characterized by electronic, IR and NMR spectral studies as well as magnetic moment and conductivity. On the basis of spectral studies, an octahedral geometry has been proposed for all the complexes. INTRODUCTION: The chemistry of macrocy...

Hexakis(2,6-diisopropylphenylisocyanide)tantalum is the first isocyanide analogue of the highly unstable Ta(CO)6 and represents the only well-defined zerovalent tantalum complex to be prepared by conventional laboratory methods. Two prior examples of homoleptic Ta0 complexes are known, Ta(benzene)2 and Ta(dmpe)3 , dmpe=1,2-bis(dimethylphosphano)ethane, but these have only been accessed via liga...

In this paper, a wireless mid-infrared spectroscopy sensor network was designed and implemented for carbon dioxide fertilization in a greenhouse environment. A mid-infrared carbon dioxide (CO₂) sensor based on non-dispersive infrared (NDIR) with the functionalities of wireless communication and anti-condensation prevention was realized as the sensor node. Smart transmission power regulation was...