BACKGROUND The aim of the current study was to assess the mRNA levels of two mitochondria-related genes, including nuclear-encoded NRF1 (nuclear respiratory factor 1), mitochondrial transcription factor A (TFAM), and mitochondrial-encoded cytochrome c oxidase subunit 1 (MT-CO1) genes in various stages of the human oocyte maturation. METHODS Oocytes were obtained from nine infertile women with...

The aims of this study were to diagnose coenurosis by means of computerized tomography (CT) scan imaging and molecular characterization of the CO1 gene using the polymerase chain reaction (PCR). Sheep and calves were necropsied, and CT scans on the cephalic region were performed on the animals. Sections of brain tissue infected with parasites were then stained with hematoxylin and eosin for mic...

A 2-year-old female donkey (Equus asinus) was euthanized in the Pathology Department of Firat University, Elazig, Turkey. Necropsy disclosed the presence of 7 hydatid cysts distributed throughout the lung parenchyma. One of those cysts represented the parasite material of the present study and was molecularly identified through sequencing of a fragment of cytochrome c oxidase subunit 1 (CO1) an...

The species status of Hyphydrus anatolicus Guignot, 1957 and H. sanctus Sharp, 1882, previously often confused with the widespread H. ovatus (Linnaeus, 1760), are tested with molecular and morphological characters. Cytochrome c oxidase subunit 1 (CO1) was sequenced for 32 specimens of all three species. Gene-trees were inferred with parsimony, time-free bayesian and strict clock bayesian analys...

The synthesis of new metal–organic coordination polymers continues to attract a great deal of interest since such materials have been shown to exhibit highly desirable solid-state properties such as magnetism, nanoporosity, second-harmonic generation and show promise for use in molecular electronics. They may also be doped with other metals to produce active heterogeneous catalysts. In particul...

A molecular phylogeographic study of Paragonimus mexicanus collected from Guatemala and Ecuador was performed. Genomic DNA was extracted from individual metacercariae, and two gene regions (partial mitochondrial cytochrome c oxidase subunit 1 (CO1) and the second internal transcribed spacer of the nuclear ribosomal gene repeat (ITS2)) were amplified by the polymerase chain reaction (PCR). Seque...

In this article we give an self contained existence proof for J. Conway’s sporadic simple group Co1 [4] using the second author’s algorithm [14] constructing finite simple groups from irreducible subgroups of GLn(2). Here n = 11 and the irreducible subgroup is the Mathieu group M24. From the split extension E of M24 by a uniquely determined 11-dimensional GF(2)M24module V we construct the centr...

Our study evaluated in silico the potential of 14 mitochondrial genes encoding the subunits of the respiratory chain complexes, including cytochrome c oxidase I (CO1), as Basidiomycota DNA barcode. Fifteen complete and partial mitochondrial genomes were recovered and characterized in this study. Mitochondrial genes showed high values of molecular divergence, indicating a potential for the resol...

Colicins Ia and Ib have been purified from mitomycin C-induced Escherichia coli strains W3110 str-r (co1 Ia) and W3110 str-r (co1 Ib) by ammonium sulfate precipitation, treatment with diethylaminoethylcellulose, and chromatography on DEAE-Sephadex, phosphocellulose, and hydroxylapatite. Sedimentation velocity and equilibrium centrifugation as well as polyacrylamide gel electrophoresis at severa...

The certainty that a species is accurately identified is the cornerstone of appearance based classification; however the methods used in classical taxonomy have yet to fully catch up with the digital age. Recognising this, the CO1 algorithm presented on the StripeSpotter platform was used to identify different species and sexes of mosquito wings (Diptera: Culicidae) and honey bee and bumblebee ...