The complement fixation test (CFT) is the only serological test prescribed by the World Organisation for Animal Health (OIE) for the diagnosis of glanders in international trading of equids. However, false-positive reactions have caused financial losses to the animal owners in the past, and false-negative tests have resulted in the introduction of glanders into healthy equine populations in pre...

Of 163 persons giving positive complement fixation tests who were exposed to mumps, 1 afterwards developed the disease; of 285 negative reactors similarly exposed, 56 afterwards came down with mumps. Of 78 individuals subjected to intimate exposure to mumps whose tests were originally negative and who failed to develop the disease, 41 per cent gave positive reactions when tested 1 month later. ...

Influenza complement fixation tests designed for use with ferret serum are described. Complement-fixing antigens derived from influenza ferret lungs were unsatisfactory due to their low content of soluble antigen; those prepared from mouse lungs or developing chick embryo membranes proved to be better antigenically and were reliable when the various reagents in the test were properly adjusted t...

However in a study of 467 lepromatous lepers, Almeida, Lima & Carvalho (1955), found the quantitative complement fixation test of Wadsworth, Maltaner & Maltaner (1938), highly specific for syphilis, when cardiolipin was used as antigen. It was pointed out that the evaluation of the specificity of the reactions, in many cases, was plagued by uncertainties, since reliable information of clinical ...

A Serological study of Q fever in human beings and animals of Pakistan was performed using complement fixation test. Sera from 56 patients having different type of infection showed that 26.8 percent had been exposed to Q fever agent and had circulating antibodies against Coxie/la burnetii. In animals ser from 55 buffaloes, 35 cows, 60 sheep, 65 goats and 300 rodents were tested and showed a pre...

The prolonged extraction of the tubercle bacillus with boiling ethyl alcohol, followed by one or more reprecipitations by chilling the hot alcoholic solution, easily yields a preparation very active as antigen in the complement fixation reaction. This preparation gives a precipitation reaction with high dilution of the normal blood serum of a number of species. The precipitation reaction presen...

Two symptomatic Toxoplasma infections of laboratory personnel have been serologically followed up for 5.5 and 10 months, respectively. Results obtained by commonly used test systems (indirect fluorescent antibody tests for IgG and IgM antibodies, complement fixation test) were compared with those of two recently developed and improved tests for IgM detection (immunosorbent agglutination assay [...

A new latex agglutination (LA) test (Wampole Laboratories, Cranbury, N.J.) for detection of antibody to herpes simplex virus was compared with a reference complement fixation (CF) method in a premarket evaluation. Of 102 serum samples tested, 19 were LA negative and CF negative, 79 were LA positive and CF positive, and 4 were LA positive and CF negative. An enzyme immunoassay (M. A. Bioproducts...

A complement fixation test, using round-bottomed microtitration plates and an 8 channel microdiluter, based on that used for brucellosis by Herr, Huchzermeyer, Te Brugge, Williamson, Roos & Schiele, 1985, has been developed for use on the sera of horses to detect antibodies to the contagious equine metritis organism. The results with 2 known positive sera tested 116 times in 27 separate tests w...