Dear Editor, In the treatment of unilateral total limbal stem cell deficiency (LSCD), conjunctival limbal autograft (CLAU) surgery taken from the healthy fellow eye with or without amniotic membrane transplantation (AMT) is one of the main therapeutic alternatives. There are some controversies regarding the optimal size of the limbal graft necessary for complete and stable epithelialization of ...

OBJECTIVE To report the use of preserved amniotic membrane and living related corneal limbal/conjunctival transplantation in total limbal stem cell and conjunctival deficiency secondary to severe Stevens-Johnson syndrome. DESIGN Prospective, noncomparative, interventional case series. PATIENTS AND METHODS Ten eyes of 10 patients with total limbal stem cell and conjunctival deficiency second...

Chemical injuries of the eye may produce extensive damage to the ocular surface epithelium, cornea, and anterior segment, resulting in permanent unilateral or bilateral visual impairment. Pathophysiological events which may influence the final visual prognosis and which are amenable to therapeutic modulation include 1) ocular surface injury, repair, and differentiation, 2) corneal stromal matri...

Lesions on the ocular surface can destroy the stem cells from the limbus and cause limbal stem cell deficiency. The limbal stem cell deficiency is marked by conjunctivalization, which can be defined as the invasion of conjunctival epithelium over the cornea. This process is accompanied by varying degrees of corneal changes such as neovascularization, inflammation, recurrent erosions, persistent...

introduction to evaluate the expression patterns of cytokeratin (k) 12, 13, and 19 in normal epithelium of the human ocular surface to determine whether k13 could be used as a marker for conjunctival epithelium. methods: total rna was isolated from the human conjunctiva and central cornea. those transcripts that had threefolds or higher expression levels in the conjunctiva than the cornea were ...

BACKGROUND Mitomycin C (MMC) and limbal conjunctival autograft (LCAU) are two known useful adjuvants in the prevention of pterygial recurrence. This study was conducted to compare the outcome of these two treatments. METHODS Prospective study on consecutive cases of primary pterygium (February 2001 to March 2002) randomised into two adjuvant groups: (1) intraoperative 0.02% MMC for 5 minutes ...

OBJECTIVE To minimize the risk to the donor eye when a conjunctival limbal autograft is performed for unilateral total limbal stem cell deficiency (LSCD), a new approach has been reported of expanding limbal epithelial progenitor cells from a small limbal biopsy cultured on amniotic membrane (AM). Herein, we present for the first time the morphologic and phenotypic outcome of one such patient. ...