Software "packages" are becoming more and more prevalent in both use and availability. These packages provide many useful functions, without the costly, time-consuming development and testing normally required to implement the desired operations. A disadvantage of many packages, however, is the difficulty in modifying them in order to receive the desired result, if the original resultant of the...

DNA sequencing can be costly and time consuming for population studies because of the relative rarity of variation along exons. These problems can be substantially reduced by the use of the polymerase chain reaction on introns using primers from the exon region. These problems can be further reduced by the use of denaturing gradient gel electrophoresis to identify those alleles in need of seque...

KEY CLINICAL MESSAGE Nonorganic vision loss (NOVL) is a relatively common condition in pediatric patients. Prompt diagnosis can prevent costly, time-consuming, and frustrating workups. It is valuable for general practitioners and specialists alike to include NOVL in their differential when evaluating patients with visual complaints that are inconsistent with normal examination findings.