55th ASMS Conference – Indianapolis 2007 www.chemistry.wustl.edu/~msf/ Overview Purposes •Evaluate the resolving power and sensitivity achievable with a new compensated trap for FTMS as a function of m/z and develop a protocol for tuning this trap. Methods •Sample empirically the cyclotron frequency surface at various cyclotron radii and z-mode amplitude distributions. •Compare the mass resolvi...

New, noninvasive methods for the early detection of urothelial carcinomas of the urinary bladder are needed for the diagnosis, follow-up, and screening of patients with bladder cancer. Detection of the enzyme telomerase in urine could offer these new diagnostic possibilities. The standard technique for detecting telomerase activity is the telomeric repeat amplification protocol (TRAP assay). Be...

Telomerase, a ribonucleoprotein, is responsible for maintaining the telomere length and therefore promoting genomic integrity, proliferation, and lifespan. In addition, telomerase protects the mitochondria from oxidative stress and confers resistance to apoptosis, suggesting its possible importance for the surviving of non-mitotic, highly active cells such as neurons. We previously demonstrated...

Environmental DNA (eDNA) assays have become a major aspect of surveys for aquatic organisms in the past decade. These methods are highly sensitive, making them well-suited monitoring rare and cryptic species. Current efforts to study Rio Grande Siren southern Texas been hampered due nature these salamanders. Arid conditions further add difficulty studying this species, as many water bodies they...

LI-IT-TOFMS (laser ionization/ion-trap storage/time-of-flight mass spectrometry) is expected to be a powerful tool for environmental monitoring. In the research reported here, real-time LI-IT-TOFMS measurements were carried out on gaseous 2-4 chlorinated PCBs in order to evaluate the applicability of an environmental monitoring method. With respect to ion-trap storage for PCBs, we found that th...

Objective: The purpose of this investigation was to determine the effect of chitosan on osteoclast cells by observing cell viability, bone resorption, and radical oxygen species (ROS) production. Methods: Osteoclast cells were obtained from the primary culture of bone marrow mouse. The osteoclast cells were identified by tartrate-resistant acid phosphatase (TRAP) marker both on the cells and th...

The aim of this study was to investigate the antioxidant activity of orange (Citrus auranthium) flesh (OF) and peel (OP) extracted with acetone, ethanol, and methanol. Antioxidant potential was examined by measuring total phenolic content (TPC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (RSA), total radical-trapping anti-oxidant potential (TRAP), oxygen radical absorbance...

OBJECTIVE This study aimed to assess the association of clinical factors with P2Y12-dependent platelet inhibition as monitored by the ratio of ADP- to TRAP-induced platelet aggregation and conventional ADP-induced aggregation, respectively. BACKGROUND Controversial findings to identify and overcome high platelet reactivity (HPR) after coronary stent-implantation and to improve clinical outcom...