نتایج جستجو برای: core gene

تعداد نتایج: 1334964  

Journal: :hepatitis monthly 0
maryam yazdanian hepatitis and aids department, pasteur institute of iran, tehran, ir iran. tel/fax: +98-2166969291 arash memarnejadian hepatitis and aids department, pasteur institute of iran, tehran, ir iran. tel/fax: +98-2166969291 ; hepatitis and aids department, pasteur institute of iran, tehran, ir iran. tel/fax: +98-2166969291 mehdi mahdavi virology department, pasteur institute of iran, tehran, ir iran. tel/fax: +98-2166496682 seyed mehdi sadat hepatitis and aids department, pasteur institute of iran, tehran, ir iran. tel/fax: +98-2166969291 fatemeh motevali hepatitis and aids department, pasteur institute of iran, tehran, ir iran. tel/fax: +98-2166969291 rouhollah vahabpour hepatitis and aids department, pasteur institute of iran, tehran, ir iran. tel/fax: +98-2166969291

conclusions results showed that hcvcp + bcg induced a moderate ctl and mixed th1/th2 immune responses with higher levels of cell proliferation and ifn-γ secretion, indicating that bcg may have a better outcome when formulated in hcvcp-based subunit vaccines. results expression and purification of core protein around the expected size (21 kda) was confirmed by western blotting. the hcvcp + bcg v...

Journal: :Journal of bacteriology 2003
Emilisa Frirdich Buko Lindner Otto Holst Chris Whitfield

The waa gene cluster is responsible for the biosynthesis of the lipopolysaccharide (LPS) core region in Escherichia coli and Salmonella: Homologs of the waaZ gene product are encoded by the waa gene clusters of Salmonella enterica and E. coli strains with the K-12 and R2 core types. Overexpression of WaaZ in E. coli and S. enterica led to a modified LPS structure showing core truncations and (w...

Journal: :iranian journal of public health 0
mr aghasadeghi sm sadat a budkowska ar khabiri s amini g bahramali

infection with hepatitis c virus (hcv) is a worldwide problem. among hcv proteins, core antigen (ag), besides its importance for diagnostic application is a prime candidate for component of a vaccine. herein, we report results of studies on production of the hydrophilic domain of core ag (2-122) in native conformation by an arabinose induction system in e.coli and the primary characterization o...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2015
Laurence Yang Justin Tan Edward J O'Brien Jonathan M Monk Donghyuk Kim Howard J Li Pep Charusanti Ali Ebrahim Colton J Lloyd James T Yurkovich Bin Du Andreas Dräger Alex Thomas Yuekai Sun Michael A Saunders Bernhard O Palsson

Finding the minimal set of gene functions needed to sustain life is of both fundamental and practical importance. Minimal gene lists have been proposed by using comparative genomics-based core proteome definitions. A definition of a core proteome that is supported by empirical data, is understood at the systems-level, and provides a basis for computing essential cell functions is lacking. Here,...

Journal: :vaccine research 0
atefeh saeedi department of microbiology, qom branch, islamic azad university, qom, iran malihe naderi department of microbiology, lahijan branch, islamic azad university, lahijan, iran alijan tabarraie golestan university of medical sciences, gorgan, iran mishar kelishdi golestan university of medical sciences, gorgan, iran amir ghaemi golestan research center of gastroenterology and hepatology (grcgh), golestan university of medical sciences, gorgan, iran, shefa neuroscience research center, tehran, iran

background: hepatitis c (hcv) is a worldwide problem without an effective vaccine with more than 170 million chronically infected people worldwide. dna vaccines expressing antigenic portions of the virus with adjutants have recently been developed as a novel vaccination technology. objectives: in the present study, a dna vaccine expressing hcv core protein was developed with il12 as a genetic a...

Journal: :FEMS microbiology letters 2011
Melissa DeYoung Michelle Thayer John van der Oost Kenneth M Stedman

Ribosomal genes are strongly regulated dependent on growth phase in all organisms, but this regulation is poorly understood in Archaea. Moreover, very little is known about growth phase-dependent gene regulation in Archaea. SSV1-based lacS reporter gene constructs containing the Sulfolobus 16S/23S rRNA gene core promoter, the TF55α core promoter, or the native lacS promoter were tested in Sulfo...

ژورنال: :پژوهش های آسیب شناسی زیستی 0
nasrin rastgoo m.sc. in medical biotechnology, tarbiat modares university, tehran, iran. ali karami ph.d. in biotechnology, associate professor, bagyatallah university, tehran, iran. farzaneh sabahi ph.d. in virology, associate professor, tarbiat modares university, tehran, iran

مقدمه: ویروس هپاتیت c یکی از مهمترین عوامل ایجادکننده هپاتیت حاد ومزمن، سیروز و سرطان کبد در سطح جهان محسوب می شود. به دلیل روند رو به رشد ابتلا به hcv در سطح دنیا تحقیقات گسترد ه ای در نقاط مختلف جهان برای افزایش کیفیت روشهای تشخیصی و تعیین ژنوتیپ ویروس در حال انجام است. روشها: در این تحقیق ناحیه ژنی مربوط به پروتئین نوکلئوکپسید ویروس (ژن core) که یک ناحیه بسیار حفاظت شده درطول ژنوم ویروس است،...

Journal: :hepatitis monthly 0
christine hartoonian department of pharmaceutical biotechnology and biotechnology research center, faculty of pharmacy, tehran university of medical sciences, tehran, ir iran; department of virology, pasteur institute of iran, tehran, ir iran zargham sepehrizadeh department of pharmaceutical biotechnology and biotechnology research center, faculty of pharmacy, tehran university of medical sciences, tehran, ir iran; department of pharmaceutical biotechnology and biotechnology research center, faculty of pharmacy, tehran university of medical sciences, tehran, ir iran. tel: +98-2164122301, fax: + 98-2166461178, e-mail: kayhan azadmanesh, department of virology, pasteur institute of iran, tehran, ir iran. tel/fax: +98-2166496682 mojtaba tabatabai yazdi department of pharmaceutical biotechnology and biotechnology research center, faculty of pharmacy, tehran university of medical sciences, tehran, ir iran yong suk jang departments of molecular biology and bioactive material sciences, institute for molecular biology and genetics, chonbuk national university, jeonju, korea lida langroudi department of virology, pasteur institute of iran, tehran, ir iran parisa amir kalvanagh department of virology, pasteur institute of iran, tehran, ir iran; department of immunology, faculty of medical sciences, tarbiat modaress university, tehran, ir iran

conclusions these results exhibited the immuno potentiator effects of mip-3beta plasmid when coadministrated with the hcv core dna vaccine. complimentary studies integrating mip-3beta as a genetic adjuvant in hcv-core-dna vaccination models are warranted. results mice covaccinated with mip-3beta elicited an enhanced th1 biased systemic immune response as evidenced by higher ifn-γ/il-4 and anti-...

Journal: :jundishapur journal of microbiology 0
sara mohammadzadeh molecular biology department, pasteur institute of iran, tehran, ir iran farzin roohvand virology department, pasteur institute of iran, tehran, ir iran soheila ajdary immunology department, pasteur institute of iran, tehran, iran parastoo ehsani molecular biology department, pasteur institute of iran, tehran, ir iran; molecular biology department, pasteur institute of iran, p. o. box: 1316943551, tehran, ir iran. tel/fax: +98-2164112219, e-mail:; ali hatef salmanian, plant biotechnology department, national institute of genetic engineering and biotechnology, p. o. box: 14965-161, tehran, ir iran. tel: +98-2144580365, fax: +98-2144580395 ali hatef salmanian agricultural biotechnology department, national institute of genetic engineering and biotechnology, tehran, ir iran; molecular biology department, pasteur institute of iran, p. o. box: 1316943551, tehran, ir iran. tel/fax: +98-2164112219, e-mail:; ali hatef salmanian, plant biotechnology department, national institute of genetic engineering and biotechnology, p. o. box: 14965-161, tehran, ir iran. tel: +98-2144580365, fax: +98-2144580395

conclusions the canola oilseeds could provide a useful expression system to produce hcv core protein as a vaccine candidate. results western blot analysis using anti-his antibody confirmed the presence of a 15 kda protein in the seeds of t1 transgenic lines. the amount of antigenic protein accumulated in the seeds of these transgenic lines was up to 0.05% of the total soluble protein (tsp). mat...

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