Peripheral human corneal epithelium grows better in tissue culture than central epithelium, but it is not known whether ocular limbal epithelium grows even better than does the peripheral corneal epithelium. In this work we compared the growth kinetics of limbal and peripheral human corneal epithelial cells in tissue culture. Four 1-2 mm2 explants, removed from the limbus or from peripheral cor...

Heterotopic grafting of murine corneas to the thoracic cage of recipient mice affords an opportunity to study the alloimmune rejection process in this well-characterized laboratory species. Immune rejection of cornea allografts can be reliably identified by direct visual, slit-lamp, and histologic observations. Virtually intact syngeneic corneal epithelium and stroma survive at the heterotopic ...

PURPOSE To investigate thickness profile changes of the corneal epithelium and Bowman's layer at the vertical and horizontal meridians with overnight myopia orthokeratology (OK) lenses. METHODS Twenty subjects (age range: 19-33 years) wore reverse-geometry rigid gas-permeable OK lenses in both eyes for 30 days. Before lens wear and after 1, 7, and 30 days of overnight lens wear, evaluation of...

The evolution of pathological changes in the corneal epithelium may be studied by tissue culture methods. We have examined the effects of conventional hard contact lenses and hydrophilic soft lenses, in order to study the effect of the direct pressure of such lenses on the corneal epithelium and the effect of prolonged oxygen deprivation on cell growth. We have also observed the time of onset o...

The survival of donor corneal epithelium was investigated in rabbits after they received unilateral 8 mm diameter lamellar keratoplasties with living donor tissue of a different sex from the host. Three weeks, 6 weeks, and 12 weeks postoperatively, cultured corneal epithelia, grown from 5 mm diameter central buttons, the adjacent 0.75 mm wide donor rings, the peripheral 10 to 13 mm diameter rin...

PURPOSE The corneal epithelium provides a barrier between the external environment and the cornea. It also serves as an ion transporting epithelium. Because of its proximity with the external environment, the corneal epithelium is frequently injured through physical or chemical insult. The purpose of this study was to determine whether corneal epithelial cell whole-cell currents change during c...

due to the high economic value of ostrich and the farming industry to further promote increased productivity, knowledge in different fields of science, including detailed knowledge of tissue structures vital organs from this animal looks. ostrich’s eye is the cornea closest to the human eye because of the same anatomical features of the human cornea, corneal transplantation can be used ostrich....

PURPOSE To determine whether topical ocular delivery of <100 nm nanoparticles can be enhanced by coating their exterior with peptide or protein ligands for cell surface receptors. METHODS A novel ex vivo bovine eye model was validated for its integrity up to 60 min. Using this model, the uptake of 20 nm polystyrene nanoparticles (administered as a single 50 mul drop) before and after surface ...

The Dkk family of secreted cysteine-rich proteins regulates Wnt/beta-catenin signaling by interacting with the Wnt co-receptor Lrp5/6. Here, we show that Dkk2-mediated repression of the Wnt/beta-catenin pathway is essential to promote differentiation of the corneal epithelial progenitor cells into a non-keratinizing stratified epithelium. Complete transformation of the corneal epithelium into a...

Insulin-depleted, normal rabbit corneal epithelium was incubated in vitro in tissue culture medium containing high concentrations of glucose (35 mM). These short-term incubations showed that the epithelium took up glucose equally whether or not insulin had been added to the medium, indicating that corneal epithelium is an insulin-insensitive tissue. Sorbitol accumulation showed that the normal ...