The multi-copper oxidases oxidise substrate molecules by accepting electrons at a mononuclear copper centre and transferring them to a trinuclear centre. Dioxygen binds to the trinuclear centre and, following the transfer of four electrons, is reduced to two molecules of water. The precise mechanism of this reduction has been unclear, but recent X-ray structural studies using the CotA endospore...

In ad hoc networks, malicious nodes can carry wormhole attacks to fabricate a false scenario on neighbor relations among mobile nodes. The attacks threaten the safety of ad hoc routing protocols and some security enhancements. We propose a classification of the attacks according to the format of the wormholes. It establishes a basis on which the detection capability of the approaches can be ide...

Tungsten-based composites have been recommended as a suitable replacement for depleted uranium. Unfortunately, one of these mixtures composed of tungsten (W), nickel (Ni) and cobalt (Co) induced rhabdomyosarcomas when implanted into the leg muscle of laboratory rats and mice to simulate a shrapnel wound. The question arose as to whether the neoplastic effect of the mixture could be solely attri...

Bacterial spores are encased in a multilayered proteinaceous shell known as the coat. In Bacillus subtilis, over 50 proteins are involved in spore coat assembly but the locations of these proteins in the spore coat are poorly understood. Here, we describe methods to estimate the positions of protein fusions to fluorescent proteins in the spore coat by using fluorescence microscopy. Our investig...

Abstract CotA laccase was successfully expressed from Pichia pastoris . Magnetic reduced graphene oxide (MRGO) nanocomposite synthesized and functionalized with iminodiacetic acid (IDA-NH 2 ), then chelated Cu 2+ for effective immobilization His-tagged laccase. The -chelated MRGO (MRGO-IDA-Cu ) showed 175 mg/g-support adsorption capacity. of MRGO-IDA-Cu nano-hybrid composite confirmed by Raman ...