The cryopreservation of mammalian embryos has become an integral part of methods to control animal reproduction. Numerous vitrification solutions have been formulated with ethylene glycol in combination with macromolecules, sugars and other cryoprotective agents. These indicate that a study of ethylene glycol as a cryoprotectant of choice in vitrification studies would be promising. To understa...

ABSTRACT Aroeira-do-sertão is a Brazilian native species that has been widely explored. Thus, the population of this reduced and techniques for its preservation are essential, such as conservation seeds in liquid nitrogen (LN). The objective work was to evaluate different cryoprotectant solutions cryopreservation aroeira-do-sertão LN (-196 °C). treatments used were: control (7.5±1.5 °C); withou...

encapsulation of olive oil is an effective method to protect it against environmental deteriorative factors. in this research, olive oil microcapsules were produced by complex coacervation method. the objective was to examine the effect of gelatin and arabic gum as shell materials, lactose as cryprotectant, and different acidification times on microencapsulation efficiency of olive oil. arabic ...

Current protocols for hematopoietic stem cell (HSC) cryopreservation are usually based on the use of 10% dimethylsulfoxide (DMSO) as an intracellular cryoprotectant with or without hydroxyethylstarch (HES) as an extracellular cryoprotectant. The toxic effects related to DMSO infusion are generally dose-related and while they are usually mild, they can become severe. HES is a relatively non-toxi...

Protein sedimentation sans cryoprotection is a new approach to magic angle spinning (MAS) and dynamic nuclear polarization (DNP) nuclear magnetic resonance (NMR) spectroscopy of proteins. It increases the sensitivity of the experiments by a factor of ∼4.5 in comparison to the conventional DNP sample preparation and circumvents intense background signals from the cryoprotectant. In this paper, w...

Biophysical characteristics of the plasma membrane, such as osmotic sensitivity and water and cryoprotectant permeability are important determinants of the function of spermatozoa after cryopreservation. A series of experiments was conducted with rhesus macaque spermatozoa at 23 degrees C to determine their: (1) cell volume and osmotically inactive fraction of the cell volume; (2) permeability ...

Cryopreservation of Senegalese sole sperm can represent an alternative to overcome some reproductive problems of this species. However, it is important to guarantee the safe use of cryopreserved sperm by selecting an appropriate protocol according to a high demand quality need to be ensured. It has been demonstrated that traditional assays such as motility and viability do not provide enough in...

Introduction Cryopreservation of the cells allows great flexible application for cell therapy, as well as industrial production of biologics such as antibody therapeutics. Conventionally, cryopreservative solution contains both of fetal bovine serum (FBS) and dimethyl sulfoxide (DMSO) as a cryoprotectant [1]. However, both of them have problems. FBS frequently induces differentiation of stem ce...

As part of an ongoing program to study the thermo-mechanical effects associated with cryopreservation via vitrification (vitreous in Latin means glassy), the current study focuses on the development of a new device for mechanical testing of blood vessels at cryogenic temperatures. This device is demonstrated on a bovine carotid artery model, permeated with the cryoprotectant cocktail VS55 and a...

We assessed the toxicity of cryoprotectant agents (CPAs) to gametophytic thalli of red alga Porphyra yezoensis at room temperature. The CPAs used were: dimethyl sulfoxide (DMSO), ethylene glycol (EG), glycerol (GC), 1,2-butanediol (1,2-BD), 1,3-butanediol (1,3-BD), 2,3-butanediol (2,3-BD), 1,3-propanediol (1,3-PD) and propylene glycol (PG). CPA concentrations of 10, 15, 20, 25, 30, 35, 40, 45, ...