1. Gall-bladder bile was obtained at cholecystectomy from seven patients who had been given [64Cu]cupric acetate intravenously. 2. Studies using dialysis and Sephadex gel filtration indicated that most of the 64Cu in the bile was in a heat-stable complex of molecular weight exceeding 50 000. The remaining biliary 64Cu was in a low-molecular-weight component. 3. Above pH 4, 64Cu could not readil...

−− The spectrophotometric method for the determination of cuprous ions in solution presented by Anwar et al. (2000), has since been analyzed and improved, especially with regard to interference from iron and ion species commonly found in acid mine drainage (AMD). The reduction of cupric ions in the presence of ferrous ions, not considered by Anwar et al., renders this method unfeasible for cupr...

The effect of cupric ions on the activity, fluorescence, and absorbance, and on the sulfhydryl and flavin adenine dinucleotide contents, of lipoyl dehydrogenase has been examined. The changes take place in two phases during the reaction of the enzyme with copper ions. In the first phase, the lipoic activity of the enzyme decreases to about 10% of its initial value, while its diaphorase activity...

To solve the main problems of existing coarse grained copper (CG Cu) intrauterine devices (IUD)-namely burst release and a low transfer efficiency of the cupric ions during usage-ultra-fine grained copper (UFG Cu) and single crystal copper (SC Cu) have been investigated as potential substitutes. Their corrosion properties with CG Cu as a control have been studied in simulated uterine fluid (SUF...

The incorporation of copper species is synthesized  in a silica matrix by the sol-gel method. We have doped copper ions and copper particles using two different copper sources: Cu(NO3)2.3H2O and copper particles chemically synthesized. We can prepare the particles of copper metallic, cuprous and cupric oxide and  compare the synthesis and characterization copper species on the silica matrix.  O...

A previously undetected pink copper protein has been isolated from human erythrocytes and purified by chromatographic procedures. The purified protein sedimented as a single symmetrical peak in the ultracentrifuge but minor impurities were detected by polyacrylamide disc electrophoresis. The apparent molecular weight was calculated to be approximately 32,000 by both Bio-Gel P-60 chromatography ...

Results of rapid reaction experiments indicate that the first observable product formed after the reaction of oxygen with purified reduced cytochrome oxidase is a spectral form which corresponds to that of the oxidized enzyme. The latter is then slowly converted to “The Compound,” or so-called oxygenated form. These observations suggest that The Compound is not an intermediate in the cytochrome...

Oxidative stability of egg and soy lecithin in emulsion was evaluated with two transition metal ions, cupric and ferric ion, at two concentration levels (50 and 500 microM). The effect of pH on lipid oxidation was also examined under these two concentrations for each ion. Egg lecithin (EL) had similar peroxide value (PV) development pattern as soy lecithin (SL) when treated with cupric ion unde...