HIV-specific cytotoxic T lymphocytes (CTLs) play an important role in the immune response to HIV infection. Long-term nonprogressors (LTNPs) or slow progressors (SPs) in HIV infection may make qualitatively different CTL responses compared to those generated by seropositive individuals who progress to disease at a faster rate. The class I molecule HLA-B*57 has been identified as one restriction...

Recently we described an unbiased bacterial whole-genome immunoinformatic analysis aimed at selection of potential CTL epitopes located in "hotspots" of predicted MHC-I binders. Applying this approach to the proteome of the facultative intra-cellular pathogen Francisella tularensis resulted in identification of 170 novel CTL epitopes, several of which were shown to elicit highly robust T cell r...

In a mixed leukocyte culture (MLC) reaction of allogenic mouse spleen cells differing for H-2K or H-2D, only a weak cytotoxic response is generated. This cytotoxic response is augmented significantly if bacterial lipopolysaccharide (LPS), 5 microgram/ml, or polyadenylic acid (poly A):polyuridylic acid (poly U), 20 microgram/ml, is present in the culture. The cytotoxic cells generated in the pre...

The cell-mediated immune response has been generated in vitro with a polyacrylamide culture system which allows the segregation of foci (clones?) of cytotoxic lymphocytes. Using the method of limiting dilutions, the frequency of precursor cells in CBA spleen cells able to generate a cytotoxic response against DBA mastocytoma is estimated at 1 per 1,700 cells.

The aim of this work is to investigate the mechanisms involved in the clearance of viral infection of the influenza virus at the epithelium level by modeling and analyzing the interaction of the influenza virus specific cytotoxic T Lymphocytes (CTL cells) and the influenza virus infected epithelial cells. Since detailed and definite mechanisms that trigger CTL production and cell death are stil...

CD8⁺cytotoxic T cells, CD4⁺ helper and regulatory cells interact differently with cancer. CD8⁺ cytotoxic recognize the antigens presented by cancer kill in various ways. recruit other immune promote them to destroy cells. On hand, suppress cell proliferation response of both This suppressive mechanism often leads progression These cell-cancer interactions can render conventional treatments inef...

The terminal step in the differentiation of those T lymphocytes that are capable of becoming cytotoxic effector cells ordinarily occurs when the effector cell precursors encounter foreign cell antigens in vivo for which they are presumably already committed . The in vitro analog for this final step, the mixed leukocyte culture (MLC)' reaction, makes possible a detailed analysis of cellular and ...

The results presented in this paper demonstrate that responding cells that remain after anti-Ia serum plus complement (C) treatment generate a highly significant in vitro cytotoxic response against minor histocompatibility complex antigens. The cytotoxic response appears to be antigen specific in that target cells of strains other than the sensitizing strain are not lysed, or lysed to a lesser ...

Severe cutaneous adverse reactions (SCARs), such as Stevens-Johnson syndrome (SJS) and toxic epidermal necrosis (TEN), are rare but life-threatening conditions induced mainly by a variety of drugs. Until now, an effective treatment for SJS/TEN still remains unavailable. Current studies have suggested that the pathobiology of drug-mediated SJS and TEN involves major histocompatibility class (MHC...