an identification of the environmental factors regulating the biosynthesis of antibiotics by fluorescent pseudomonads is an essential step toward improving the ability of these bacteria to control plant pathogens. investigation of nine fluorescent pseudomonads, utilized in growth suppression of rhizoctonia solani, the causal agent of root rot and damping-off of bean plants, by the method of hpl...

The aim of this microcosm study was to determine influence of the antibiotic 2,4 diacetylphloroglucinol (DAPG) on the effect of wild type and functionally modified Pseudomonas fluorescens F113 strains in a sandy loam soil of pH 5.4 planted with pea (Pisum sativum var Montana). The functional modification of strain F113 was a repressed production of DAPG, useful in plant disease control, creatin...

Bacterial extracellular electron transfer (EET) is envisioned for use in applied biotechnologies, necessitating electrochemical characterization of natural and engineered electroactive biofilms under conditions similar to the target application, including small-scale biosensing or biosynthesis platforms, which often distinct from standard 100 mL-scale stirred-batch bioelectrochemical test platf...

Pseudomonas fluorescens Q8r1-96 produces 2,4-diacetylphloroglucinol (2,4-DAPG), a polyketide antibiotic that suppresses a wide variety of soilborne fungal pathogens, including Gaeumannomyces graminis var. tritici, which causes take-all disease of wheat. Strain Q8r1-96 is representative of the D-genotype of 2,4-DAPG producers, which are exceptional because of their ability to aggressively coloni...

Prokaryotic regulatory proteins respond to diverse signals and represent a rich resource for building synthetic sensors and circuits. The TetR family contains >10(5) members that use a simple mechanism to respond to stimuli and bind distinct DNA operators. We present a platform that enables the transfer of these regulators to mammalian cells, which is demonstrated using human embryonic kidney (...

The genotypic diversity of antibiotic-producing Pseudomonas spp. provides an enormous resource for identifying strains that are highly rhizosphere competent and superior for biological control of plant diseases. In this study, a simple and rapid method was developed to determine the presence and genotypic diversity of 2,4-diacetylphloroglucinol (DAPG)-producing Pseudomonas strains in rhizospher...

Seed treatment with a 2,4-diacetylphloroglucinol (DAPG)-producing Pseudomonas strain ameliorated abiotic stress disorder in corn caused by growth in a low-pH soil. In two consecutive growing seasons, Wood1R-treated seed gave rise to plants that grew taller (P<or=0.05), had fewer foliar lesions (P<or=0.10), and provided greater yields (P<or=0.1) than the negative controls when grown in soil with...

Dark pigmented fungi of the Gaeumannomyces-Phialophora complex were isolated from the roots of wheat grown in fields in eastern Washington State. These fungi were identified as Phialophora spp. on the basis of morphological and genetic characteristics. The isolates produced lobed hyphopodia on wheat coleoptiles, phialides, and hyaline phialospores. Sequence comparison of internal transcribed sp...