A sensitive fluorimetric method was developed for the quantitative determination of DNA in plant (Zea mays L. and Medicago sativa L.) extracts. This method takes advantage of the specific increase in fluorescence intensity of the complex of DNA and the dye 4',6'-diamidino-2-phenylindole (DAPI). Recovery of DNA and dissociation of histones from DNA were maximized by the addition of 2.0 molar NaC...

Ultracentrifuging (400,000 g for 4-6 h at 4 degrees C) living wild-type cells of the fission yeast Schizosaccharomyces pombe moves the nucleus towards the ends of the cells but scarcely affects their viability. However, in the long cells produced by growing the mutant cdc2.33 for 4-6 h at the restrictive temperature (36.5 degrees C), ultracentrifuging (as above) gives an intense fluorescence wi...

The fluorescent dye 4,6-diamino-2-phenylindole (DAPI) has previously been used to label starburst amacrine cells selectively in the rabbit retina and AII amacrine cells in the cat retina. Using the rabbit retina, we show that intraocular injection of DAPI labels starburst amacrine cells as seen 1-2 days later. In contrast, after a brief in vitro incubation with DAPI, AII amacrine cells are sele...

We cloned genes, designated smdAB, that encode a multidrug efflux pump from the chromosomal DNA of clinically isolated Serratia marcescens NUSM8906. For cells of the drug-hypersensitive strain Escherichia coli KAM32 harboring a recombinant plasmid carrying smdAB, structurally unrelated antimicrobial agents such as norfloxacin, tetracycline, 4',6-diamidino-2-phenylindole (DAPI), and Hoechst 3334...

Spirogyra nucleoli were shown by three-dimensional optical microscopy of DAPI fluorescence to contain DNA with a pattern and distribution matching those of the fibrillar centres. This was confirmed using different species with nucleoli showing different sizes of fibrillar centre. Much lower levels of fluorescence were seen corresponding to the dense fibrillar component. Nearly all the DAPI fluo...

Current methods to measure the fraction of active glycosylase molecules in a given enzyme preparation are slow and cumbersome. Here we report a novel assay for rapidly determining the active fraction based on molecular accessibility of a fluorescent DNA minor groove binder, 4',6-diamidino-2-phenylindole (DAPI). Several 5,6-dihydrouracil-containing (DHU) DNA substrates were designed with sequenc...

Hindgut symbiotic trichomonads (uninucleate Caduceia versatilis, and multinucleate Stephanonympha sp. and Snyderella tabogae) from the dry-wood-eating termite Cryptotermes cavifrons (Kalotermitidae) accumulate DAPI (4,6diamidino-2-phenylindole) in the membranous sacs of the Golgi complex. This form of Golgi complex, typical of protists in the class Parabasalia, is called a parabasal body. Trich...

Three different techniques were applied to analyze the patterns of longitudinal chromosome differentiation in five Sesbania species (S. exasperata, S. punicea, S. sesban, S. tetraptera, and two different geographical populations of S. virgata). For all the species investigated, the prophase chromosome condensation was always proximal. After staining with the fluorochromes chromomycin A, and DAP...

In this study, a detailed chromosome analysis of the endemic Cappadocian Chub, Squalius cappadocicus in Melendiz Stream (Aksaray) was performed. The standard Giemsa staining, C-banding (CBG and CB-DAPI), Ag-NOR technique were applied. diploid number 50; its karyotype formula 14M + 16Sm 10St 10A. Heteromorphic sex chromosomes weren’t detected studied specimens. all chromosomal arms (NF) 90. C-ba...

Abstract Background Pressurized Intra-Peritoneal Aerosol Chemotherapy (PIPAC) is an innovative treatment against peritoneal carcinomatosis. Doxorubicin a common intra-venous chemotherapy used for carcinomatosis and PIPAC. This study evaluated the impact of increased PIPAC intraperitoneal pressure on distribution cell penetration doxorubicin in sheep model. Methods was aerosolized using into cav...