Decellularization offers great potential to the field of tissue engineering, as this method gives rise to scaffold material with the native organ architecture by removing all cellular material and leaving much of the extracellular matrix (ECM) intact. However, many parameters may affect decellularization efficacy and ECM retention and, therefore, decellularization protocols need to be optimized...

BACKGROUND The dermis is a commonly used source tissue for biologic scaffolds; all cellular and nuclear materials need to be removed to limit the inflammatory immune response by the host organism. The decellularization is critical because it must preserve the structural integrity of the extracellular matrix. This work has analyzed a decellularization procedure commonly followed for the dermal t...

Bladder replacement or augmentation is required in congenital malformations or following trauma or cancer. The current surgical solution involves enterocystoplasty but is associated with high complication rates. Strategies for bladder tissue engineering are thus actively sought to address this unmet clinical need. Because of the poor efficacy of synthetic polymers, the use of bladder acellular ...

Introduction Perforation of maxillary sinus mucous membrane is of the most prevalent complication during open sinus lift surgery. Moreover, such complication can usually be managed by an absorbable membrane. As far as absorbable membranes are concerned, decellularized maxillary sinus mucous membranes, which is an extracellular matrix, can be used as a biologic scaffold and insulating membrane ...

Diaphragmatic impairments exhibit high morbidity as well mortality while current treatment options remain unsatisfactory. Tissue engineering (TE) approaches have explored the generation of an optimal biocompatible scaffold for diaphragmatic repair through tissue decellularization or de novo construction, with without addition cells. The authors conducted a systematic review on state art in (DTE...

An attempt has been made to formulate a new method of corneal decellularization using a direct perfusion system through the cornea. Here, we compared the direct perfusion method to some commonly used decellularization strategies including chemical methods; non-ionic detergent TRITON X-100 and ionic detergent; sodium dodecyl sulphate (SDS) based orbital shaker method and physical methods of liqu...

OBJECTIVE During the last decade, face allotransplantation has been shown to be a revolutionary reconstructive procedure for severe disfigurements. However, offer to patients remains limited due to lifelong immunosuppression. To move forward in the field, a new pathway in tissue engineering is proposed. BACKGROUND Our previously reported technique of matrix production of a porcine auricular s...

introduction perforation of maxillary sinus mucous membrane is of the most prevalent complication during open sinus lift surgery. moreover, such complication can usually be managed by an absorbable membrane. as far as absorbable membranes are concerned, decellularized maxillary sinus mucous membranes, which is an extracellular matrix, can be used as a biologic scaffold and insulating membrane i...