نتایج جستجو برای: dehalogenases

تعداد نتایج: 191  

Journal: :Acta Crystallographica Section A Foundations and Advances 2017

Journal: :Annals of Microbiology 2021

Abstract Purpose Bacillus megaterium strain BHS1, isolated from an alkaline water sample taken Mavi Gölü (Blue Lake, Turkey), can grow on minimal medium containing 2,2-dichloropropionic acid. We characterized this bacterium at the genomic level. Methods The HiSeq platform was used to carry out genome sequencing, de novo assembly, and scaffolding with BHS1. Next, data were analyzed demarcate DNA...

Journal: :Applied and environmental microbiology 2011
Khomaini Hasan Andrea Fortova Tana Koudelakova Radka Chaloupkova Mayuko Ishitsuka Yuji Nagata Jiri Damborsky Zbynek Prokop

We report the biochemical characterization of a novel haloalkane dehalogenase, DatA, isolated from the plant pathogen Agrobacterium tumefaciens C58. DatA possesses a peculiar pair of halide-stabilizing residues, Asn-Tyr, which have not been reported to play this role in other known haloalkane dehalogenases. DatA has a number of other unique characteristics, including substrate-dependent and coo...

Journal: :Philosophical transactions of the Royal Society of London. Series B, Biological sciences 2013
David Leys Lorenz Adrian Hauke Smidt

Bacterial respiration has taken advantage of almost every redox couple present in the environment. The reduction of organohalide compounds to release the reduced halide ion drives energy production in organohalide respiring bacteria. This process is centred around the reductive dehalogenases, an iron-sulfur and corrinoid containing family of enzymes. These enzymes, transcriptional regulators an...

Journal: :Biochemistry 2002
Aaron J Oakley Zbynek Prokop Michal Bohác Jan Kmunícek Tomás Jedlicka Marta Monincová Ivana Kutá-Smatanová Yuji Nagata Jirí Damborský Matthew C J Wilce

The hydrolysis of haloalkanes to their corresponding alcohols and inorganic halides is catalyzed by alpha/beta-hydrolases called haloalkane dehalogenases. The study of haloalkane dehalogenases is vital for the development of these enzymes if they are to be utilized for bioremediation of organohalide-contaminated industrial waste. We report the kinetic and structural analysis of the haloalkane d...

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