When human plasma is mixed with testosterone-(3)H and subjected to electrophoresis on paper in glycine acetate buffer at pH 8.6, at least two proteins other than albumin bind the testosterone. In normal women 80.5 +/- 1.9% (SEM) of the recovered radioactivity migrates with the beta-globulins, 7.3 +/- 0.80% with the inter-alpha-globulins, and 4.3 +/- 0.40% with albumin. In normal men the percent...

I t was demonst ra ted in a preceding s tudy from these laboratories tha t pat ients with the genetic liver disease acute in termi t ten t porphyr ia (AIP) 1 display a significant defect in the reductive t ransformation of prototy~)e steroid hormones such as testosterone and dehydroisoandrosterone (1). This defect is manifest by the marked preferential metabol ism of these hormones along the 5 ...

The principal urinary 17-ketosteroids (17KS) were analyzed in 16 cases of hirsutism; their conjugated forms were separately cleaved, and the liberated 17KS were chromatographed and quantitated. Six were clinically classified as cases of Stein-Leventhal syndrome and the remaining 10 as "idiopathic hirsutism." 1 The following conditions were excluded a priori: adrenal and ovarian tumors, Cushing'...

A variety of 5beta steroid metabolites derived from hormones natural to man are potent inducers experimentally of delta-aminolevulinate synthetase, the rate-limiting enzyme in porphyrin-heme formation. This mitochondrial enzyme is found at high levels of activity in the livers of patients with the genetic disease, acute intermittent porphyria (AIP). In this study the metabolism of (14)C-labeled...

SIMPLE, RAPID TEST-TUBE EXIRACTION TECHNIC for the determination of urinary 17-ketosteroids has seen developed in our laboratories. A complete urinary 17-ketosteroid determination was carried out in a glass stoppered photometer cuvet by combining this extraction technic with a colorimetric method. Three colorimetric methods, Zimmermann (1), (2), Pincus (3), and Cohen and Kaluszyner (4) were com...

The present paper reports a method for determining the respective concentrations of total, o(-, and /3-17-ketosteroids in urine which is based upon the previously reported method of assaying mixtures of crystalline androsterone (a-steroid) and dehydroisoandrosterone (p-steroid) (1) and a simple means of eliminating the interfering effect of the non-ketonic substances from the calorimetric deter...