We monitored bacterioplankton communities from Chesapeake Bay over 2 years (2002–2004) by use of denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S rRNA gene. Chesapeake Bay bacterioplankton exhibited a repeatable annual pattern and strong seasonal shifts. In winter, the bacterial communities were dominated by Alphaproteobacteria and Actinobacteria, whereas in summer, the predo...

Recirculation technology has been emerging in the marine aquaculture industry. The microbiome developed recirculation systems (RASs) is an important factor for optimal operation of these and fish welfare. In this study, microbial community dynamics water column biofilms a RAS with Mediterranean species gilthead sea bream bass were investigated, while physicochemical conditions also monitored. M...

Mezcal is the second most important alcoholic beverage produced in Mexico, and microorganisms present during fermentation process are primarily responsible for its organoleptic characteristics. Among these, bacterial populations have been less studied, mainly due to difficulty of recovering them. The aim was apply a culture-independent molecular technique detect involved spontaneous mezcal Dura...

Abstract The hydrogen content of the two sludge samples namely TDS and PLS were increased after heat shock pretreatment from 239.23 ± 10.23 mL/L to 663.87 15.99 at 80°C 173.61 17.21 496.74 30.21 60°C, respectively. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) indicated that Enterococcus Clostridium dominant bacteria in consortium. Two strains successfully isolate...

Oil palm plantations are the most invasive land use changes in Southeast Asia. It must have affected unique natural biodiversity. This study aimed to investigate diversity of soil bacteria based on 16S rRNA gene profiles from tropical forest and oil plantation Jambi Province, Indonesia. Soil sample was taken province, The site is Bukit Duabelas National Park, nearby Sarolangun District, bacteri...

In the present study, a comprehensive, rapid and sensitive method for screening sequence variation of the human mitochondrial tRNA genes has been developed. For this purpose, the denaturing gradient gel electrophoresis (DGGE) technique has been appropriately modified for simultaneous mutation analysis of a large number of samples and adapted so as to circumvent the problems caused by the anomal...

A one-step denaturing gradient gel electrophoresis (DGGE) strategy for the rapid detection of mutations in the factor VIII gene of haemophilia A patients is described. All coding (except the middle part of exon 14) and flanking intronic regions of the gene corresponding to approximately 6.6 kb were amplified in 27 fragments using four PCR programs. Heteroduplex formation was performed for each ...

Denaturing Gradient Gel Electrophoresis is a influential and feasible device for sequence examination of the variety of complicted natural microbial populations. For PCR amplification of 16S rDNA products and specific primers were used and analyzed by denaturing gel electrophoresis in gradient. Probes specific for the cluster Genusand were designed to bind to sequences within the region amplifi...

Polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) fingerprinting was recently introduced into food microbiology. This paper describes the technique and reports on the state-of-the-art application of this technique to food and food-related ecosystems. Applications of PCR-DGGE in several fields of food microbiology are reviewed: the identification of microorganisms isol...