Eighty-seven strains of Staphylococcus aureus were examined for their ability to produce enterotoxins A, B, and C, deoxyribonuclease, lysozyme, proteinase, lipase, and a-, ,B-, and 6-hemolysins. Enterotoxigenic strains showed a significant tendency to be high lipase producers, but none of the other enzymes formed were correlated with the ability of the staphylococci to produce enterotoxins A, B...

Of the four deoxyribonucleases, DNases A, B, C, and D, observable by chromatography of preparations of the pancreatic enzyme on cellulose phosphate, DNase D, the component present in the smallest amount, has not been previously characterized. Peptide maps show that the sequence of the amino acid residues in the polypeptide chain of DNase D is indistinguishable from that of DNase C; analysis of ...

A methyl green decolorization micro test for the determination of streptococcal anti-deoxyribonuclease B titers in serum is described. The micro test was compared with an alcohol precipitation macro test on 112 human sera. The anti-deoxyribonuclease B titers agreed within one dilution step for 85.7% of the 112 sera examined by both techniques. Both tests were about equal in the detection of tit...

As a result of chance observations, the authors studied for deoxyribonuclease activity 16 strains of Salmonella, including six fresh isolates and ten stock cultures, with positive results in 13. Reactions characteristically occurred at 48 hours or later, with the majority being manifest at 72 hours and the latest at six days. No other positive reaction was observed among approximately 7,000 Ent...

The behavior of developing anthers has been studied with respect to deoxyribonuclease. This enzyme, in contrast to related hydrolytic ones, is sharply periodic in its activity. Whenever a pool of deoxyribosides appears in situ, it is preceded by the appearance of deoxyribonuclease. The duration of pool or enzyme does not exceed and is generally less than 12 hours, even though the life span of t...

Porcine spleen DNase II, a lysosomal acid hydrolase, is a noncovalently linked azb heterodimer (Liao, T.-H. (1985) J. Biol. Chem. 260, 10708–10713). The a subunit, after disulfide cleavage, yields two chains, a1 and a2. The complete amino acid sequences of the a1, b, and a2 chains were elucidated by protein sequencing, and the pairings of one interchain disulfide between a1 and a2 and of three ...

Eighty-seven strains of Staphylococcus aureus were examined for their ability to produce enterotoxins A, B, and C, deoxyribonuclease, lysozyme, proteinase, lipase, and alpha-, beta-, and delta-hemolysins. Enterotoxigenic strains showed a significant tendency to be high lipase producers, but none of the other enzymes formed were correlated with the ability of the staphylococci to produce enterot...