The yield of Aspergillus niger mycelium from a synthetic medium can be increased by the addition of microgram quantities of cholesterol, ergosterol, cholestanol, 7-dehydrocholesterol, stigmasterol, sitosterol, pregnenolone, and the vitamins D. The stimulation is not due to degradation to the acetate level. It is obtained only in highly aerated cultures. The rate of growth of Torula utilis was n...

EXPERIMENTAL hypertensive cardiovascular disease is easily induced in the rat by desoxycorticosterone acetate (DCA) especially, although by no means exclusively, in animals sensitized by augmented sodium intake and removal of a kidney.' Other mineralocorticoid steroids, differing quantitatively in potency, have the same effect.' Depending upon the circumstances of the experiment, either benign ...

After the synthesis of desoxycorticosterone (DOC), it became apparent that this corticosteroid, the physiological effects of which are concerned primarily with sodium and potassium metabolism, can produce a state of hypertension both in animals and in man (1, 2). The induced hypertension is dependent upon the administration of adequate amounts of dietary sodium, whereas the hypertensive state, ...

Patients with lipemic nephrosis, cardiac failure, and hepatic cirrhosis frequently accumulate an excess of extracellular fluid, distributed in a pattern characteristic of the underlying disease. These patients commonly have a marked impairment of sodium excretion, while glomerular filtration may be low, normal, or even increased (1-4). This impairment of sodium excretion suggested the presence ...

The desexycorticosterone-mimetic action of ammoniated glycyrrhizin on electrolyte and water metabolism and on pituitary-adrenal function, was demonstrated in rats. Retention of sodium and water was observed in both intact and adrenalectomized rats during 4 hour minerosteroid assays, the degree of retention being a function of log dose. In intact rats, the antidiuretic effect of glycyrrhizin was...

Chronic exhibition of chicks to desoxycorticosterone acetate produces a slight but definite rise in blood pressure. This effect is increased by addition of salt to the diet. This regimen does not affect cholesterol levels and atherogenesis in the chick on ordinary mash. However, in the presence of a mildly atherogenic diet (0.25 per cent cholesterol) concomitant DCA exhibition increases the inc...

Excised guinea pig ciliary body-iris preparations accumulated <C-labeled L-ascorbate. The concentrating mechanism required glucose as well as Na+, K+, and Ca++ ions in the media and is inhibited by ouabain, dinitrophenol, iodoacetate, cyanide, fluoride, desoxycorticosterone, and hydrocortisone. It demonstrates saturation kinetics with increasing concentrations of Lascorbate in the media and is ...

The influence of alterations of adrenal function upon the responsiveness of the rat to intravenously administered ouabain was studied electrocardiographically by the string galvanometer. Conduction disturbances followed by cardiac arrest were the criteria for a definitive response. Adrenalectomized rats showed a significant increase in sensitivity to ouabain as compared with normal controls. No...

Adrenalectomized dogs maintained on desoxycorticosterone acetate developed a fatal circulatory collapse when given normal dog plasma. They showed general restlessness, a rapid fall in blood pressure and cardiac index, a decline in central venous pressure and general prostration. Most dogs developed a bloody diarrhea and a minority showed edematous swelling of the face with itching. Animals with...