【Introduction】 8-Oxo-2 ́-deoxyguanosine (8-oxo-dG) is a representative nucleoside damage that is formed by oxidation of 2 ́-deoxyguanosine (dG) with reactive oxygen species (ROS), and its presence has been linked to aging, cancer, etc [1]. Unlike dG, 8-oxo-dG forms stable base pairs with both 2 ́-deoxycytidine (dC) and 2 ́-deoxyadenosine (dA). Based on the base-pairing properties of 8-oxo-dG, DNA p...

We have solved the structures of the homoduplex d(Gm5CGCGCG)2, and the heteroduplexes d(GCGCGCG)/d(TCGCGCG) and d(GCGCGCG)/d(CCGCGCG). The structures form six base-pairs of identical Z-DNA duplexes with single nucleotides overhanging at the 5'-ends. The overhanging nucleotide from one strand remains stacked and sandwiched between the blunt-ends of two adjacent Z-DNA duplexes, while the overhang...

Proton 2D-NOE spectroscopy has been used to investigate the three-dimensional conformations of several sonicated polydeoxynucleotides in solution. The observed pattern of cross peaks indicate that poly(dAdT) . poly(dAdT1 in all salt concentrations studied (up to 6.6M CsF), and poly(dG-m5dC) . poly(dG-m5dC) in low salt (0.1M NaCU are righthanded B-structures. Poly(dG-m5dC) . poly(dG-m5dC) in Mg2...

10.1601/nm.2199 CLST is an extremely acidophilic gamma-proteobacteria that was isolated from the Gorbea salt flat, an acidic hypersaline environment in northern Chile. This kind of environment is considered a terrestrial analog of ancient Martian terrains and a source of new material for biotechnological applications. 10.1601/nm.2199 plays a key role in industrial bioleaching; it has the capaci...

چکیده: حضور منابع تولید پراکنده (dg) در سیستم قدرت در مقابل مزایای اقتصادی آن، می­تواند باعث ایجاد عدم هماهنگی در عملکرد رله­های اضافه جریان گردد. در این مقاله شاخص جدیدی، مستقل از تنظیمات رله­ها، به منظور تعیین میزان تأثیر نصب dg بر قیود هماهنگی رله­های اضافه جریان ارائه گردیده است. در صورت حضور منابع تولید پراکنده در شبکه، جریان خطای عبوری از رله­های پشتیبان و اصلی نسبت به حالت عدم حضور آن من...

The binding of ethidium bromide (EtdBr) to the dC-dG-dC-dG self-complementary duplex has been monitored at the resolvable drug and nucleic acid protons and backbone phosphates at high nucleotide/drug (N/D) ratios by nuclear magnetic resonance (NMR) spectroscopy in aqueous solution. We observe averaged resonances (25 degrees-95 degrees) for the nucleic acid and drug nonexchangeable protons in th...

The carcinogen 2-acetylaminofluorene forms two major DNA adducts: N-(2'-deoxyguanosin-8-yl)-2-acetylaminofluorene (dG-AAF) and its deacetylated derivative, N-(2'-deoxyguanosin-8-yl)-2-aminofluorene (dG-AF). Although the dG-AAF and dG-AF adducts are distinguished only by the presence or absence of an acetyl group, they have profoundly different effects on DNA replication. dG-AAF poses a strong b...

Dystroglycan (DG or DAG1) is considered a critical link between the basement membrane and the cytoskeleton in multiple tissues. DG consists of two subunits, an extracellular α-subunit that binds laminin and other basement membrane components, and a transmembrane β-subunit. DG-null mouse embryos die during early embryogenesis because DG is required for Reichert's membrane formation. DG also form...