The effects of cycloheximide and 5-fluorouracil on the formation of low-molecular-weight ribonucleic acid (RNA) in yeast were investigated. Both compounds were found to affect the synthesis of low-molecular-weight RNA of ribosomal origin more than transfer RNA but less than the high-molecular-weight ribosomal RNA. 5-Fluorouracil-containing transfer RNA was separated from normal transfer RNA by ...

disease can be distinguished from one another and from control human polymerases by their elution behavior upon diethylaminoethyl cellulose chromatography and Sephadex gel filtration, by the magnesium ion concentration for optimal activity, by the stimulatory or inhibitory effects upon enzymatic activity of monovalent cations, and by sensitivity of enzymatic activity to heparin inhibition. Thes...

Nuclei were isolated by hypotonie shock and homogenization from freshly drawn, nonstimulated leukemic leukocytes and were pulse labeled with deoxythymidine triphosphate-3H. Labeled oligodeoxyribonucleotides were separated from the bulk of DNA and proteins by membrane cone filtration and were further purified by alkaline hy drolysis and diethylaminoethyl cellulose chromatography. In parallel exp...

By utilizing conventional techniques of pressure ultrafiltration, gel filtration chromatography, diethylaminoethyl cellulose chromatography, and preparative polyacrylamide electrophoresis, the A component of the group D lysin produced by Streptococcus zymogenes has been purified to a state of apparent homogeneity when determined by the techniques of anionic and cationic disc gel electrophoresis...

The B protein, a subunit of lactose synthetase (EC 2.4.1. c), was crystallized from bovine skim milk and bovine mammary tissue. The B protein was identified as cu-lactalbumin, based on the following criteria: substitution in the enxymic rate assays, spectra, immunological titrations, amino acid composition, mobility on starch gel electrophoresis, molecular weight, and cochromatography on diethy...

The incorporation of glucosamine-'4C by Ehrlich ascites tumor cells has been mess ured both in vivo and in vitro. A considerable amount of high molecular weight radio active material appears outside of the cells under both conditions. The radioactive extracellular fluids were subjected to gel ifitration and to chromatography on DEAE cellulose (diethylaminoethyl cellulose) columns. These experim...

Three serologically distinct antigens were identified in homogenates of Saint Louis encephalitis (SLE) virus-infected cells after Brij-58 solubilization and diethylaminoethyl (DEAE)-cellulose chromatography. These antigens were designated as antigen I, II, and III. Immunodiffusion analyses showed that all antigen peaks which eluted from the DEAE-cellulose column contained the intracellular majo...

Cyclic nucleotide phosphodiesterase activity was studied in 33 malignant neoplastic, 2 benign neoplastic, and 18 nonneoplastic human mammary tissues. Enzyme activity, using both cyclic adenosine 3':5'-monophosphate and cyclic guanosine 3':5'-monosphosphate as substrates, was measured in whole homogenates over a concentration range of 1 to 100 muM. Specific activity was calculated at substrate c...

Human erythrocyte glucose 6-phosphate dehydrogenase (o-glucose 6-phosphate :nicotinamide adenine dinucleotide phosphate oxidoreductase, EC 1.1.1.49) was purified by column chromatography with diethylaminoethyl cellulose, calcium phosphate gel, carboxymethyl cellulose, diethylaminoethyl Sephadex, and carboxymethyl Sephadex. A homogeneous preparation was obtained in over-all yield of about 50%. T...