نتایج جستجو برای: dige
تعداد نتایج: 682 فیلتر نتایج به سال:
An essential step in 2D DIGE-based analysis of differential proteome profiles is the accurate and sensitive digitalisation of 2D DIGE gels. The performance progress of commercially available charge-coupled device (CCD) camera-based systems combined with light emitting diodes (LED) opens up a new possibility for this type of digitalisation. Here, we assessed the performance of a CCD camera syste...
Hyperphosphorylated keratin (K) 8 acts as a phosphate "sponge" for stress-activated protein kinases thereby inhibiting pro-apoptotic molecules and thus apoptosis. MAP kinase/ERK1 has increased activity in colorectal cancer (CRC) and is known to phosphorylate K8. The aims were to identify the K8 isoforms abundantly present in colon tumors, using 2D difference gel electrophoresis (DIGE), to ident...
• Difference gel electrophoresis (DIGE) is the new gold standard analysing complex protein mixtures in proteomics. It is used for measuring the expression levels of proteins in different mixtures on the same two-dimensional electrophoresis (2-DE) gel. In this paper we review a method for the calibration and normalization of those protein expression measurements. Further we show how to find trea...
سابقه و هدف: استئوآرتریت (OA) شایعترین بیماری دژنراتیو مفصلی و مهمترین علت ناتوانی بیماران مسن میباشد. آسیب غضروف یک مشکل عمده در بیماری استئوآرتریت است. در این مطالعه بهمنظور فهم پاتوژنز استئوآرتریت به بررسی پروتئوم کندورسیت غضروف بیماران مبتلا به استئوآرتریت در مقایسه با غضروف نرمال پرداختهایم. مواد و روشها: در این مطالعه پروتئینهای غضروف 7 بیمار مبتلا به استئوآرتریت در مرحله نهائی، که...
Protein expression profiles in rat bladder smooth muscle were compared between animal models of streptozotocin-induced diabetes mellitus (STZ-DM) and age-matched controls at 1 week and 2 months after induction of hyperglycemia with STZ treatment. At each time point, protein samples from four STZ-DM and four age-matched control rat bladder tissues were prepared independently and analyzed togethe...
2DE and 2D-DIGE based proteomics analysis of serum from women with endometriosis revealed several proteins to be dysregulated. A complete list of these proteins along with their mass spectrometry data and subsequent bioinformatics analysis are presented here. The data is related to "Investigation of serum proteome alterations in human endometriosis" by Dutta et al. [1].
Distinguishing between benign follicular thyroid adenoma (FTA) and malignant follicular thyroid carcinoma (FTC) by cytologic features alone is not possible. Molecular markers may aid distinguishing FTA from FTC in patients with indeterminate cytology. The aim of this study is to define protein abundance differences between FTC from FTA through a discovery (proteomics) and validation (immunohist...
OBJECTIVES To identify the most significant salivary biomarkers in Sjögren's syndrome (SS) using proteomic methods. METHODS Parotid saliva from 20 non-SS subjects and 41 primary SS patients was analysed. Protein expression profiles for each sample were generated by surface-enhanced laser desorption/ionization time-of-flight-mass spectrometry (SELDI-TOF-MS). Mean peak intensities of SS patient...
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