whether fasting and changes in diet might be changing leptin levels, thus altering the function of T cells. The implication of this new work is that leptin drives the activity of pro-inflammatory, self-reactive T cells, and that starvation, which reduces leptin production, changes the pattern of cytokines generated and the disease-inducing potential of the T cells. So, the authors show that ins...

In order to improve simultaneous detection and identification of Helicobacter genus in general and Helicobacter pylori specifically and reduce the number of amplifications needed, we established a multiplex PCR. In this study, two pairs of primers: Hcom1 and Hcom2 specific for Helicobacter genus, Hicd1 and Hicd2 specific for Helicobacter pylori species were used. To determine the sensitivity of...

Background: Taq DNA polymerase is a very important enzyme for molecular biological studies such as DNA amplification and DNA sequencing by the PCR. It is a standard enzyme that is used in 90% of molecular biology labs today. The aim of this study was to produce Taq DNA polymerase enzyme in E. coli by a reliable, practical, simple and low cost method. Materials and Methods: In this study, the T...

identification of host blood meal in haematophagous arthropods is an important element in their rule in transmission of vector borne diseases. the effects of post ingestion and physical conditions that killed mosquitoes are stored on the success of detecting blood meal dna of anopheles stephensi and culex quinquefasiatus was investigated by polymerase chain reaction (pcr) amplification at the h...

Isothermal DNA amplification is an alternative to PCR-based amplification for point-of-care diagnosis. Since the early 1990s, the approach has been refined into a simple, rapid and cost-effective tool by means of several distinct strategies. Input signals have been diversified from DNA to RNA, protein or small organic molecules by translating these signals into input DNA before amplification, t...

A sensitive DNA isothermal amplification method for the detection of DNA at fM to aM concentrations for pathogen identification was developed using a non-stick-coated metal microfluidic bioreactor. A portable confocal optical detector was utilized to monitor the DNA amplification in micro- to nanoliter reaction assays in real-time, with fluorescence collection near the optical diffraction limit...

This study aimed to establish a method for the selective amplification of cell-free fetal DNA (cffDNA) in maternal plasma and preserve the integrity of DNA fragments during amplification, thereby providing a sufficient amount of cffDNA to meet the requirement of routine non-invasive prenatal testing. We amplified DNA molecules in a one-reaction system without considering their particular sequen...

Demand for herbal products has increased because of their purported health benefits and economic value. However, they are susceptible to adulteration, making accurate identification origin essential, especially quality control. In recent decades, DNA-based methods have played a crucial role in the development authentication tools that required good DNA. The manufacturing process involved heatin...

Polymerase chain reaction is the most widely used method for in vitro DNA amplification. However, it requires thermocycling to separate two DNA strands. In vivo, DNA is replicated by DNA polymerases with various accessory proteins, including a DNA helicase that acts to separate duplex DNA. We have devised a new in vitro isothermal DNA amplification method by mimicking this in vivo mechanism. He...